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The PAG and Related Genes Expression in the Blood of Cows during Early Pregnancy

Author: LvLian
Tutor: WangXingLong
School: Yangzhou University
Course: Animal Genetic Breeding and Reproduction
Keywords: Dairy cow Pregnancy diagnosis PAG Semi-quantitative PCR mRNA differential display
CLC: S823
Type: Master's thesis
Year: 2009
Downloads: 67
Quote: 1
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Abstract


Dairy cattle breeding , as soon as possible to determine the pregnancy or not , and strengthen management in order to have the pregnancy , to find out why not pregnancy , and catch up on with or necessary treatment . This is of great significance to shorten the the cows calving interval , and improve their fecundity . This experiment were the use of a semi-quantitative PCR technology and mRNA differences in technology , research the cows blood PAG gene 's expression amount as the basis of early pregnancy diagnosis methods as well as empty pregnant cattle and pregnancy bovine blood -related gene expression of differences at the molecular level understand the variation of gene expression in early pregnancy diagnosis process , provide a reference to the new method for the diagnosis of early pregnancy in mammals . The results are as follows: the PAG gene expression -pregnant cattle and bovine blood pregnancy of 21 days of gestation , pregnancy the bovine PAG gene expression amount slightly higher than the empty pregnant cattle , but the difference was not significant , t = 2.3695 lt ; t0.05 ( 34 ), P gt; 0.05. This also shows that the breeding after 21 days cows blood the PAG gene expression can not be used as the basis for early pregnancy diagnosis . Screening in the blood of pregnant cows to 6 , respectively, the specific expression of the fragment 3 with known functional genes or regulatory genes similar sequencing and comparative analysis of the remaining bands of unknown genes or similar genes . : S1 sequence and transforming growth factor TGF-β2 has high homology ( 98% ) , TGF-β2 bovine trophoblast and endometrial epithelial hyperplasia promote epithelial cell growth and dumped into trophoblast inhibition ; TGF-β2 the endometrial transformation variation and livestock epithelial promote growth . S4 sequence and sets barbed drop factor 1 has high homology (97%), CSF can stimulate the growth of early placental , can inhibit NK cell function , and to reduce the incidence of abortion . The the S5 sequence with the insulin-like growth factor with high homology (98%), IGF can stimulate the placental prolactin release , regulate other steroid hormone synthesis and secretion by membrane receptors . IGFs with blastocyst implantation , placenta formation and function , fetal growth and development , promotion of trophoblast invasion conducive to embryo implantation ; involved in the regulation of numerous aspects of early pregnancy , the embryonic development of important factors .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Cow
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