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Study on Preparation of High-quality Wheat Bran Dietary Fiber and Their Monosaccharide Compositions and Properties

Author: WangLi
Tutor: XiaoAnHong
School: Wuhan Polytechnic University
Course: Cereals, Oils and Vegetable Protein Engineering
Keywords: High - quality dietary fiber Cellulose enzyme Xylan enzymatic Extrusion Monosaccharide composition analysis Properties of
CLC: TS210.1
Type: Master's thesis
Year: 2009
Downloads: 299
Quote: 2
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Abstract

The topics to wheat bran as raw material, the conditions on the use of different methods of preparation of high quality dietary fiber, and monosaccharide composition and properties of dietary fiber samples prepared by different methods of measurement and comparative analysis. The best experimental conditions of cellulose enzyme preparation of high-quality wheat bran dietary fiber: solid-liquid ratio 1:10, enzyme dosage 20U / g, pH 5.0, temperature 40 ° C, enzymatic hydrolysis time 4h, SDF / IDF = 1 : 3.06 high-quality wheat bran dietary fiber. The optimum conditions for xylanase enzymatic preparation of high-quality wheat bran dietary fiber: hydrolysis temperature 70 ℃, enzymatic time 2h, enzyme dosage 15U / g, solid-liquid ratio 1:15, SDF / IDF = 1: 3.07 high-quality wheat bran dietary fiber. Most suitable experimental parameters squeeze Preparation of high quality wheat bran dietary fiber: material moisture content of 11.6%, the extrusion temperature of 80 ° C, screw speed 100r/min the screw length three sections, get SDF / the IDF = 1:3.09 high quality wheat bran dietary fiber. Monosaccharide composition analysis using high performance liquid chromatography (HPLC) were prepared by different methods of high quality dietary fiber results show that: the samples of the original wheat bran, cellulose enzyme preparation of dietary fiber samples and extrusion prepared dietary fiber samples The main component of arabinoxylan and cellulose and hemicellulose xylan glucuronic acid, accounting for 77% -80%, and 11% -15% of the poly-galactose and 6% -10% fruit poly sugar; Enzymatic Preparation of the xylanase component of the 85.22% of the dietary fiber samples arabinoxylan and xylan, glucuronic acid, and 9.84% poly-galacturonic and 3.93% of fructan. Dietary fiber holding hydraulic, oil-absorbing ability, expansion force, soluble substance content, and cation exchange capacity, Nitrite adsorption capacity of cholesterol adsorption capacity, viscosity, and particle size distribution and other properties of the sample were measured. The results show that: extrusion prepared samples of dietary fiber, cellulose enzymatic preparation of samples of dietary fiber and xylan enzymatic preparation of dietary fiber sample holding capacity than the original samples of wheat bran to improve 136%, 132% and 126%, oil absorption capacity were increased by 238%, 231% and 213%; expansion force of all the appropriate granularity respectively 28.9%, 24.7% and 18.7%, swelling force first decreases with decreasing sample size and then increases; soluble matter content increases with increasing temperature is almost the same at the same temperature, the soluble substance content of four samples; poor cation exchange capacity of the enzymatic preparation of dietary fiber samples, extrusion processing of dietary the influence of the cation exchange capacity of the fiber is not obvious; at a pH of 2, the sample is a strong adsorption of nitrite ion; xylanase Enzymatic Preparation of dietary fiber sample, extrusion dietary fiber samples prepared, cellulose Enzymatic Preparation of dietary fiber samples at pH7, the amount of adsorption of the cholesterol is 7.82mg / g, 7.48mg / g, 7.39mg / g, while the original wheat bran samples to 4.96mg / g, under a neutral condition (analog The adsorption capacity of the adsorption capacity of the pH environment of the small intestine) were higher than that under acidic conditions (simulated gastric acidic conditions); gradually increases in viscosity with increasing concentration of the sample solution, but an increasing trend gradual, extrusion Preparation of the viscosity of the sample is large, the smaller of the original wheat bran samples, at a solution concentration of 5%, and its viscosity were 13.9mPa · s and 12.5MPa · s; Enzymatic Preparation of dietary fiber samples and extrusion 73% -75% of the particle size of the dietary fiber prepared samples distributed in the LT; 160 mesh range, while about 70% of the particle size of the original wheat bran samples distributed in GT; 180 mesh range. Scanning electron microscope observation results show that: the original dietary fiber sample particles, surface roughness, structural linkages very dense; the cellulose enzymatic preparation of dietary fiber sample surface rendering of large and small granular, smaller pore structure remains relatively tightly; Enzymatic Preparation of the xylanase dietary fiber sample is flaky, indirectly, have a layered structure of the spatial network, and the network structure is no rule; extrusion prepared dietary fiber structure of the sample is destroyed, more serious, its different shapes, sheet, there are smaller particles, the structure is relatively loose, more pore.

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