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Genetic Analysis and QTL Mapping on Several Important Agronomic Traits of the Wheat Backbone Parent Mazhamai

Author: DuXiPeng
Tutor: CheYongHeï¼›GuoXiaoMin
School: Hebei Normal University of Science and Technology
Course: Crop Genetics and Breeding
Keywords: Backbone Parental Genetic Analysis Genetic mechanisms Parent selection QTL mapping Merit gene cluster
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 23
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Abstract


Backbone Parental Combining Ability and polygenic coordination expression played an important role in the cultivation of new varieties of crops. So far, China has wheat Backbone Parental bred many excellent varieties of wheat. Backbone Parental grasshopper wheat and other varieties of jasper wheat hybrid breed superior varieties of Bima 1-6, Bima used to promote up to million mu, Bima 4 is important wheat backbone of one of the parent These fine varieties derived a lot of the material has an important value. Grasshopper wheat can be nurtured by what is the genetic basis of a large number of fine varieties? Current urgent problems. Backbone Parental grasshopper wheat and other varieties of jasper wheat parent, get whichever hybridization, 185 F2 plants build groups, groups of plant height, effective tillers, panicle length, spikelet number, grain number genetic analysis and QTL mapping and grain weight, help provide a reference for revealing the molecular genetic mechanisms of Backbone Parental matching and improve the breeding efficiency of wheat crossbreeding parents. Grasshopper wheat, and jasper wheat and F2 phenotypic data analysis found that grasshopper wheat plant height and grains per spike significantly better than jasper wheat. F: population, plant height lower than the mid-parent value individuals accounted for 80.54%, higher than the mid-parent value of individual grains per spike accounted for 63.24%, plant height and grain number at the same time better than in pro-value individuals accounted for 47.03%, This shows that the grasshopper wheat plant height and grain number have made great contributions to future generations. Pro this grasshopper wheat and jasper wheat genome-wide scan, total screening 2,899 pairs of SSR, EST-SSR and STS cited material, including Xgwm Xgdm Xbarc Xwmc Xcfa, Xcfd, Xcfe, Swes, cnl, Dupw, For , Ksum and Mag Series, 455 pairs of primers were polymorphic between the parental average screening rate 15.70% Xgwm, and Xbarc series primers effects best screening rate is higher than 30%, while the EST-SSR and STS primers normality lower. Emphasis on chromosome 1A, 1B, 2A, 2B, 5A, 5B and 6B plant height, effective tillers, panicle length, spikelet number, grain number and grain weight QTL mapping, involving 96 pairs of primers, were detected in 9 a QTL, QTL loci distributed on five chromosomes 2B and 6B chromosome outside: 3 chromosome 1A above, 1B and 2A above two each, 5A and 5B, each one. The five main effect QTL, which are: effective tillers three located in 1A, 1B and 5A chromosomes; grain weight two are located in 1A chromosome. Most of the QTL dominance effects are positive, their effects come from the female parent grasshopper wheat, so these QTL loci, grasshopper wheat contribution of genetic effects on offspring. 4. Bima 4 Backbone Parental compared with the previous found in previous studies of wheat chromosome important section of this study found that the QTL on chromosome 1A and 5A sites: 1A chromosome -Xwmc278 within the QTL loci related to the effective number of tillers and grain weight QTL loci are located predecessors found backbone parent Bima 4 alleles high frequency genetic the section Xgwm357-Xbarc 119-Xgwm135; 5A chromosome QTL sites related grains per spike is closely related to important segments found in previous Bima 4 grain weight traits Xgwm415-Xcfa2250-Xbarc117-Xbarc186-Xgwm304 described in 1A and 5A chromosomes does The presence of the the excellent gene cluster, this may be one of the important reasons for the grasshopper wheat to become the the wheat backbone of the pro-this.

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