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Alkaloid of tobacco is also known as nicotine, belonging to pyridine alkaloids. Nicotine is the main alkaloid in tobacco, which is a central nervous stimulator, and addictive substance after taking, causing physical and psychological dependence. Microbiology are used to degrade nicotine in tobacco, this approach will not cause the loss of incense smoke and can also improve gas quality、increase resource utilization value of tobacco, it can also reduce the damage of nicotine to human health, and lessen large amounts of waste generated in the tobacco industry on the ecological environment. Therefore, this study will have great development and application potential. Tobacco soil from liuyang of Hunan province was used as an experimental material. One microbiological strain was screened and selected to see the effectivity of the degrading nicotine. Then identification of the optimum growth and degrading of nicotine could be studied to provide a basis for the microbiology in practice.(1) Tobacco soil from liuyang was used as experimental material to screen strains degrading nicotine, their rate of degrading nicotine wre determined by UV spectrophotometer after the stains were cultured 2、4、6 days. The best one which can degrade nicotine effectively is z1-3-3-1, its degration is 72.48% in 48h.(2) Firstly the strain z1-3-3-1 was identificated from its Morphology, for example its colony morphology and individual morphology, then it was identificated from the molecular level, extracting its DNA.electrophoresis check, PCR amplification.electrophoresis check and homologous gene comparison to Clearly identified that strain’s Properties.(3) The strain was cultured in different carbon source、Nitrogen source、pH、temperature、nicotine concentration and inoculum concentration to determine its best growth conditions, the result is that nicotine concentration 0.1%, temperature 28℃, pH7.0, rotate speed 120 r/m, inoculum concentration 8%, Sodium citrate concentration 2.0%, peptone concentration 1.0%.
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