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Identification of High Virulence of Metarhizium Against White Grubs and the Field Application Research of New Formulations

Author: ShenJianFei
Tutor: YinYouPing
School: Chongqing University
Course: Biology
Keywords: Metarhizium anisopliae Grubs Microgranule Milk powder agent Mixture
CLC: S476.1
Type: Master's thesis
Year: 2011
Downloads: 70
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Abstract


Beetle the CPI pest larvae, commonly known as white grubs, are a class of important soil pests, and so far the studies suggest that the prevention and treatment of one of the more difficult pests. Grubs and pests harmful to crops the underground vegetative organs, and has a certain degree of secrecy. Peanut is an important oil crops and food crops, has significant economic value. However, in recent years, grubs and pests in the main producing areas of Shandong, Henan and other peanut crop hazards worsening trend year by year, coupled with the large-scale use of chemical pesticides, not only polluted the soil and the environment but also severely reduce the yield of peanuts quality. Metarhizium anisopliae is an important class of biocontrol fungi, has been widely used in the production of the world there are about 200 types of insects can be different strains of Metarhizium lethal infection. The Metarhizium potential for prevention and control of soil pests has been many biocontrol expert attention. This study from Shandong Rizhao, Qingdao the peanut production demonstration Ota acquisition naturally diseased peanuts the grubs in cadaver isolated two Metarhizium anisopliae strain the numbered CQM132 and CQM135. The taxonomic status of the strains were identified and measured in the indoor virulence of two strains of peanut grubs. 2009-2010, from April to September in Rizhao City, Shandong Province, Town of acres of pollution-free peanut production base set randomized block cell Field trials using Metarhizium anisopliae CQMa128 fine granules and milk powder agent in peanut sowing time and The young fruit grubs control effect and impact of biodiversity on the field peanut testing and evaluation. Indoor Metarhizium complex formulation suitable for the control of soil pests peanut grubs preliminary study. biology culture morphological characteristics, identification results show that the strains are CQM132 and CQM135 to Metarhizium anisopliae (Metarhizium anisopliae). ② bioassay, the strains CQM132 and CQM135 peanut grubs (Anomala corpulenta and the dark gills beetles larvae) have strong virulence. Strains CQM132 high concentration of spore suspension (1.1 × 107 spores / ml-1.1 × 108 spores / ml) are up more than 85% of the cumulative mortality of the two beetles larvae, the LT50 ≤ 7.58d lethal. A high concentration of the strain CQM135 spore suspension (1.1 x 107 spores / ml-1.1 x 108 spores / ml) of two beetles larvae cumulative mortality reached 90% or more the LT50 of ≤ lethal 6.85d. In contrast, the virulence of the the strains CQM135 two beetle larvae slightly better than strains CQM132, has a good prospect of application in the peanut field. ③ in the spring and peanut sowing time application of one billion spores / g Metarhizium the bacteria CQMa128 sustained release granules (optimal dose 375kg/hm ~ 2), after application of 30-40d, the average correction control efficiency of 75.58% to 80.03% . The same period survey Health zone the peanut surrounding the natural enemies of grubs - hook Bumblebee did not have obvious adverse effects. ④ In the the spring peanut fruitlet period application of 10 billion spores / g Metarhizium anisopliae (optimum dose of the bacteria CQMa128 milk powder agent 7.5kg/hm ~ 2), after application of 15-20d, the average correction control effect reached 77.59% to 86.46%. Highest field average fruit retention rate in the high dose Metarhizium formulations in the treatment area from 85.76% to 87.13%, the peanut pods fresh weight average maximum increase production reached 832.5 ~ 916.4kg/hm, with considerable chemical pesticides, controlling pests Paul produced a significant effect. The same period survey Health zone the peanut surrounding the natural enemies of grubs - hook Bumblebee did not have obvious adverse effects. ⑤ Green anisopliae CQMa128 and the CQM135 of pure pollen with low toxicity and high chemical pesticides cypermethrin, imidacloprid, and chlorpyrifos has good compatibility. Indoor bioassay, of Metarhizium CQMa128 and optimal efficiency and low toxicity CQM135 with three chemical insecticides complex ratio in the range of 1% -1.5%.

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CLC: > Agricultural Sciences > Plant Protection > A variety of control methods > Biological control > The use of microbial pathogens
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