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Study of Diversity of Trichoderma Isolated from East China

Author: LiGuangJi
Tutor: ChenJie；LiuLiXing
School: Shanghai Jiaotong University
Course: Plant Pathology
Keywords: Polymorphism Classification Chitinase T.harzianum Peptaibols
CLC: S476
Type: Master's thesis
Year: 2011
Downloads: 44
Quote: 0
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Abstract


Trichoderma (Trichoderma spp.) Has a wide range of applications in the field of agriculture, especially in industry, agriculture, environmental protection. The International already on the registration of more than 100 species of Trichoderma biocontrol agents or bacterial fertilizer products and commercial production, played an important role in agricultural production. Trichoderma diversity study has comprehensively reveal the of Trichoderma biocontrol mechanism and ecological functions of great value. Antagonistic strains of Trichoderma isolated from different ecological regions resources, screening with different characteristics, and study its diversity has important implications for the protection and rational use of Trichoderma resources. Were found 89 species of Trichoderma and our total of 24 species. Carry out the Trichoderma resource survey and diversity of ecological zones in the East China has been unmanned systems. In this study, 287 soil samples were collected from the East China 1026 separation Trichoderma. Trichoderma were identified 13 species, among which the Trichoderma koningii T. koningiopsis newly recorded species in China, the Harz Trichoderma Trichoderma populations of East China dominant species. Studied the geographic distribution of the 13 Trichoderma and biological characteristics of diversity and the diversity of some strains of antimicrobial peptides and the Trichoderma harzianum intraspecific genetic diversity. The results show that: the Trichoderma in general have significant ecological, biochemical, genetic diversity. In this study, AFLP (amplified fragment length polymorphism) technology as well as four genes: ech42, chit33, tef1α, the ITS analysis of the T. harzianum intraspecific genetic diversity of T. harzianum intraspecific genetic diversity is very high . For the kinds of species heterogeneity and other biocontrol research data. The differences between different species of Trichoderma chitinase and mainly antagonistic spectrum of secondary metabolites, the foundation for the establishment of high-throughput screening of Trichoderma repository and the biocontrol strains. The results showed that: 13 kinds of Trichoderma main types of chitinase enzymatic activity has a larger difference between chitinase activity and antagonistic activity of Trichoderma closely linked. Trichoderma can produce a variety of secondary metabolites of antagonistic class, which Peptaibols class of antimicrobial peptides Trichoderma produce antimicrobial effect is very important secondary metabolites. This study was used in conjunction for high performance liquid chromatography and mass spectrometry (HPLC / MS) detection of the six better antagonistic Trichoderma the Peptaibols class of antimicrobial peptides, were detected 16 kinds of Peptaibols classes of antimicrobial peptides, found that strains in the antimicrobial peptide species there are differences. Laid the foundation for further study of the diversity of antimicrobial peptides and mechanism of action of Trichoderma.

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CLC: > Agricultural Sciences > Plant Protection > A variety of control methods > Biological control
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