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Correlation of hMLH1, hMSH2 and XRCC3 Gene Expressions to Endometrial Adenocarcinoma and Its Significance

Author: WangYan
Tutor: DengYanJie
School: Dalian Medical University
Course: Obstetrics and Gynaecology
Keywords: endometrial adenocarcinoma DNA mismatch repair hMLH1 hMSH2 XRCC3 homologous recombination repair
CLC: R737.33
Type: Master's thesis
Year: 2009
Downloads: 62
Quote: 1
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Abstract


Objective:Endometrial cancer is epithelial malignant tumors from the endometrial glands ,which is one of three malignant tumors in female genital tract, accounting for 7% of women systemic tumors, and about 20%? 30% of malignant tumors of female reproductive system. In recent years, its incidence in the world tended to rise. With the a full realization of this disease, endometrial cancer diagnosis and treatment have been some changed ,operation is still main means, but radiotherapy, chemotherapy, biological treatment and (or) some useful supplements, such as hormone therapy integrated treatment are also play an important roles . However, a number of the advanced( above I step , more than uterine body tumor) ,the relapsed patients and the women with high risk factors cannot have unsatisfactory results by those treapies.Therefore, the study of the develop- ment process of endometrial cancer will provides the basis for early diagnosis. Researchs suggest that the incidence of endometrial cancer and other tumors, is a complex mechianism involved in multi-factor and multi- ple genes, including inactivation of tumor suppressor gene, activation of oncogenes, as well as defection of a variety of DNA replication and repair gene .The relationship between the occurrence and development of endo- metrial cancer has become a hotspot in recent years. It have been recognized by the majority of researchers that DNA mismatch repair and homologous recombination genes paly the role in the development of endometrial adenocarcinoma ,but the interaction between genes ,the way they play their function and the results of the interaction is still not clear at present. In this experiment, DNA mismatch repair and homologous recom- bination repair as the two aspects of DNA repair system may play a role in the occurrence of endometrial adenocarcinoma development. The ex- pressions of the productions of mismatch repair genes hMLH1, hMSH2 and homologous recombination repair gene XRCC3 between normal endo- metrium and endometrial adenocarcinoma tissues, may reveal the incidence of endometrial adenocarcinoma mechanism and provide the evidence of diagnosis of early adenocarcinoma.Methods:From January 2007 to May 2008 in Dalian hospital for gynecology and obstetrics, 46 cases of endometrial adenocarcinoma, which is diagnosed without radiation, chemotherapy, 14 cases of normal endo- metrium ( 9 cases of proliferative phase, 5 cases of secretory phase). Through the method of immunohistochemistry , observing the expressions of the products of mismatch repair genes hMLH1, hMSH2 and homologous recombination repair gene XRCC3 in normal endometrium, endometrial adenocarcinoma tissues. The data was analyzed by SPSS 17.0, and examin- ed the correlation between expressions of hMLH1, hMSH2 and XRCC3 genes between tumor grade, operation?pathological stage and lymph node metastasis relevance.Results:1. Under microscopy hMLH1 positive expression is mainly in the cytoplasm and some nucleolus. hMLH1 in endometrial adenocarcinoma with low expression rate, the positive expression rate of 67.39%, significantly lower than that of endometrial tissue to 100%. In endometrial adeno- car- cinoma ,the expressions of hMLH1 in tumor grade, operation?patho- logic stage and lymph node metastasis have no correlation2. Under microscopy hMSH2 positive expression mainly in the nucleus and some cytoplasm. hMSH2 in endometrial adenocarcinoma with low ex- pression rate, the positive expression rate of 60.86%, significantly lower than that of endometrial tissue to 100%. In endometrial adenocarcinoma expressions of hMSH2 in tumor grade, operation?pathologic stage and lymph node metastasis have no correlation3. Under microscopy XRCC3 positive expression mainly in the cyto- plasm and some nuclei. XRCC3 endometrial adenocarcinoma in high expression, the positive rate of 97.82%, significantly higher than that in normal endometrial tissue The positive rate of 35.71%. In endometrial adenocarcinoma expressions of XRCC3 in tumor grade, operation?patho- logic stage and lymph node metastasis have no correlation.Conclusion: In endometrial adenocarcinoma the expression of DNA mismatch repair genes hMLH1 and hMSH2 have some degree of deficiency, comparing with the expression rate of normal endometrial tissue;the expres- sion of homologous recombination gene XRCC3 in endometrial adeno- carcinoma was significantly higher than that in normal endometrial tissue. These changes may be the markers of early-warning function in occurrence and development of endometrial adenocarcinoma.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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