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Study on the Loss of Heterozygosity on Chromosome 3,7,8,9,17 in Clear Cell Renal Carcinoma

Author: QinJie
Tutor: LiQuanLin
School: Dalian Medical University
Course: Urology
Keywords: Loss of heterozygosity (LOH) Renal clear cell carcinoma Tumor suppressor gene (TSG)
CLC: R737.11
Type: Master's thesis
Year: 2009
Downloads: 21
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Abstract


Objective: development of kidney cancer is a multi-stage, multi-step process, including oncogene activation and tumor suppressor genes (tumor suppressor gene, TSG) inactivation, including a series of genetic alterations. Deletion or inactivation of tumor suppressor gene is a tumor of one of the important molecular events in the development process. Therefore find associated with a particular type of tumor TSG and research the inactivation status change is important. Tumors often appear in the tumor suppressor gene loci chromosomal gene showed allele loss of heterozygosity (loss of heterozygosity, LOH) LOH analysis of tumors by detecting its laws can be found in the chromosomes within a certain range of tumor suppressor genes. Of this study was to detect allelic loss of heterozygosity (LOH), renal cell carcinoma, providing clues to find the tumor suppressor gene associated with renal cell carcinoma. Methods: 3,7,8,9,17 on chromosome 10 microsatellite polymorphism flag D3S1038, D3S1234, D3S1300, D3S1317, D3S1540, D3S1597, D7S522, D8S261, D9S171, TP53, 25 cases of clear cell renal cell carcinoma organizations and their corresponding normal kidney tissue for PCR amplification, polyacrylamide gel electrophoresis and ethidium bromide staining and detection of genomic DNA of LOH, and to study the relationship of LOH with different pathological stage classification of renal cell carcinoma. Results: 25 patients with renal cell carcinoma in 23 cases (23/25, 92%) of at least appear in a site LOH, which the the P16 gene D9S171 incidence of up to 47.37% (9/19), followed by Caveolin-1 gene D7S522 locus LOH rate of 34.78% (8/23), the P53 gene TP53 LOH was 30% (6/20), VHL gene D3s1038, D3s1317 LOH incidence D3s1597 sites were 29.41% (5/17), 25% (6/24) and 26.67% (4/15), of FHIT gene D3s1300, D3s1234 D3s1540 sites LOH incidence of 29.41% (5/17), 19.05% (4/21) and 10% (1/10). FEZ1 gene D8S261 LOH incidence of 13.01% (3/23), the staggered renal clear cell carcinoma LOH occurs the difference was not statistically significant (Χ ~~ 2 = 0.2 P = 0.655, Χ ~~ 2 = 4.269 P = 0.092). Conclusion: The renal clear cell carcinoma prevalent allele loss of heterozygosity, suggesting that the VHL gene FHIT gene, Caveolin-1 gene, P16 gene, FZE1 gene and P53 gene may be associated with the occurrence of cc-RCC development is closely related. Different pathological stage classification of renal cell carcinoma whose LOH difference was not statistically significant suggesting that LOH occurrence may be an early event in the development of renal cell carcinoma.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
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