Dissertation > Excellent graduate degree dissertation topics show

Apoptosis and Mechanism Study of EGCG Induced Human Uterine Cervix Cancer HeLa Cells

Author: TanZuo
Tutor: LuoZhaoYang
School: Nanhua University
Course: Pathology and Pathophysiology
Keywords: Epigallocatechin-3-gallate HeLa cell line cellular apoptosis Caspase-3 protein PRAP-1 protein
CLC: R737.33
Type: Master's thesis
Year: 2011
Downloads: 34
Quote: 0
Read: Download Dissertation

Abstract


Objective: Uterine cervix cancer is one of common malignant tumour and seriou threaten national femme healthy. Chemotherapy elevated remission rate of uterine cervix cancer in middle-advanced stage, but the curative effect of part patients wasn’t satisfactory because of secondary effect of chemotherapy drug and drug fast of tumour cell. To investigate mechanism of Epigallocatechin-3-gallate （EGCG） induced apoptosis of uterine cervix cancer HeLa cells and provid experimental information for development and search new drug.Methods: MTT method detected 50% inhibiting concentration （IC50） that EGCG inhibted HeLa cellular growth. The growth of HeLa cell inhibited by EGCG was observed through the cellular growth curve, colony formation and light microscope. The cellular apoptosis of EGCG induced HeLa cells were checked by Hochest33258 staining, flow cytometry （FCM） and DNA gel electrophoresis. The expression of Caspase-3 gene and protein was respectively analyzed by RT-PCR, immuocytochemistry and Western- blotting. Meanwhile, the expression of active Caspase-3 protein and spallation of PRAP-1 protein was detected by Western- blotting. In addition, the expression alteration of PRAP-1 protein between nucleus and endochylema of HeLa cells treated with EGCG was observed by immuocytochemistry.Results: 1. IC50 that EGCG inhibted the growth of HeLa cells was 36.82 mg·L-1. 2. The results of cellular growth curve, colony formation and light microscope showed that EGCG depressed growth of HeLa cell was increased with it concentration increased and treatment time extended （n=3, P<0.05）. 3. The result of Hochest 33258 staining displayed that the typical morphology characteristic of cell apoptosis example nuclear fragmentation and karyopyknosis occurred in HeLa cell. The number of cellular apoptosis increased with EGCG concentration increased （n=3, P<0.05）.4. The result of flow cytometry （FCM） displayed that the apoptosis rate of HeLa cell gradually increase with EGCG concentration increased and EGCG induced HeLa cell apoptosis for concentration dependent it （n=3, P<0.05）. 5. The result of DNA gel electrophoresis showed that DNA ladder strap was detect in HeLa cell treated by EGCG and it is more obviously with its concentration increased. 6. The results of RT-PCR discovered that the expression of Caspase-3 gene in HeLa cells obviously degraded with EGCG concentration increased （P<0.05）. 7. The western-blotting results exhibited that expression of Caspase-3 protein in HeLa cells obviously increased with EGCG concentration increased （P < 0.05） and EGCG induced expression of Caspase-3 protein for concentration dependent it. 8. The results of immuocytochemistry exhibited that the expression of Caspase-3 protein lay in cytoplasm and EGCG induced expression of Caspase-3 protein with its concentration increased （P<0.05）. 9. The western-blotting results showed the expression of active Caspase-3 protein and spallation of PRAP-1 protein was increased by EGCG , depend on its concentration, in HeLa cells （P<0.05）. 10. The results of immuocytochemistry discovered that the expression of PARP-1 protein was in nucleus and endochylema of HeLa cells. With EGCG concentration increased, the expression of PARP-1 protein was up-regulated in endochylema and down-regulated in nucleus （P<0.05）.Conclusion:1. EGCG inhibited the growth of uterine cervix cancer HeLa cells to depend its concentration.2. EGCG can induce HeLa cellular apoptosis in vitro.3. EGCG depressed the expression of Caspase-3 gene and protein in HeLa cells to accelerate the spallation of PRAP-1 protein and to provoke the cellular apoptosis and inhibited the growth of HeLa cells.

Related Dissertations

  1. EGCG Promoting Apoptosis of Human Prostate Cancer Cell PC-3 and Its Influence on the Expressions of Survivin Protein,R737.25
  2. Effect of Epigllocatechin Gallate on Synaptic Transmission in Hippocampus in Normal Rats and Cerebral Ischemia-Reperfusion Rats,R285.5
  3. The Study of Imatinib Mesylate on Radiosensitivity of Human Cervical Cancer Cell Line Hela under Normal and Hypoxia Growth Conditions,R737.33
  4. Livin, Caspase-3 and MVD in bladder carcinoma and its significance,R737.14
  5. Radioprotective Effect and Mechanism of Nitroxides R-1,R965
  6. DAURICINE active ingredients on human lung cancer cell line A549 induced apoptosis and anti-proliferative effects of experimental study,R734.2
  7. The in Vitro Reversing Effect of Arsenic Trioxide on Tumor Immunosuppression from Uterine Cervix Cancer Hela Cells,R737.33
  8. Effect of EGCG on Expression of RECK and Its Modulating Mechanism in Gastric Cancer Cell Line MGC803,R735.2
  9. Expression and Significance of Livin and Capase-3 in Epithelial Ovarian Cancer and Their Correlation,R737.31
  10. Effects of Flavonoids from Astragalus Complanatus and Epigallocatechin-3-gallate on Carbon Tetrachloride-induced Hepatic Fibrosis in Mice and Their Mechanisms,R285.5
  11. Studies on the Anti-tumor Action and the Mechanism of Active Components of Menispermum Dauricum,R285
  12. Retinal and Systemic Side-Effects Studies Following Intravitreal Injection of Bevacizumab (AVASTIN) in Adult Mice,R774
  13. Protective Effects and Mechanisms of EGCG on APP/PS1 Transgenic Mice,R749.16
  14. Expression of the HIC-1 in Human Cervical Carcinoma Cell Line Hela and Its Regulation by Methylation,R737.33
  15. Study on Antitumor Action of "Xiaoliutang" Recipe,R285
  16. Experimental study of phlegm method on neuronal apoptosis in Alzheimer disease cell model,R285.5
  17. Expression and Significance of PTEN and Caspase-3 in Vulvar Neoplasia,R737.35
  18. Study of the Expression of Caspase-3 in Human Normal Esophageal Epithelium, Dysplasia, Esophageal Carcinoma, Metastasis Lymph Node and the Relationship between Its Expression with Apoptosis,R735.1
  19. Apoptosis and its related protein p53 , bcl - 2 , caspase - 3 expression in human thyroid carcinoma and Hashimoto's thyroiditis and significance,R581.4
  20. Effects of Antisense Oligodeoxynucleotides Targeting Hypoxia Inducible Factor 1α on the Proliferation and Apoptosis in Glioma Cell Line U251,R739.4

CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
© 2012 www.DissertationTopic.Net  Mobile