Dissertation > Excellent graduate degree dissertation topics show

Proteomics Analysis of the TGF-beta Response Program in GBM Cells

Author: ZhangWeiYi
Tutor: ZhengShu；LinBiaoYang
School: Zhejiang University
Course: Bioinformatics
Keywords: SILAC Triple-SILAC Tandem mass spectrometry Proteomics Glioblastoma TGF-beta
CLC: R739.4
Type: Master's thesis
Year: 2011
Downloads: 75
Quote: 0
Read: Download Dissertation

Abstract


TGF-beta Plays an essential role in maintaining tissue homeostasis through its ability to induce cell cycle arrest, differentiation, apoptosis, and to preserve genomic stablity.Thus, TGF-beta is a potent anticancer agent that prohibits the uncontrolled proliferation of epithelial, endothelial and hematopoietic cells. Interestingly, tumorigenesis typically elicits aberrations in TGF-beta signaling pathway that engenders resistance to the cytostatic activities of TGF-beta, thereby enhancing the development and progression of human malignancies. Moreover, these genetic and epigenetic events conspire to convert TGF-beta from a suppressor of tumor formation to a promoter of their growth, invasion and metastasis. TGF-beta regulates tumorigenesis and its signaling pathways are often modified during tumor progressionin human cancers, such as Glioblastoma (GBM), which is the most common and most aggressive malignant primary brain tumor in human. Prior to initiation and early during progression TGF-beta acts as a tumor suppressor, however, at later stage it is often a tumor promoter. The dichotomous nature of TGF-beta during tumorigenesis is known as its paradox.Here, we focus on the potential role of TGF-betal in Glioblastoma, and proteomics analysis of the TGF-beta response in GBM cells by a novel method: Triple-SILAC. We have developed an efficient workflow by combining this method and other traditional molecular methods. The applicability of some new methods in this workflow was proved. The intracellular proteins and extracellular matrix proteins from GBM cell line M059J were extracted for SILAC experiments separately. As results,2771 intracellular proteins and 471 extracellular matrix proteins were identified by MS/MS. Within these proteins,255 intracellular proteins and 93 extracellular matrix proteins with significant differential expression between the GBM samples of TGF-betal+/-treatment were found by SILAC, which might be associated with TGF-beta response in GBM cells. Furthermore, the quantitative change of mRNA for 19 proteins chosed from those 348 proteins was validated by qPCR. In conclusion, our results provide a valuable protomics dataset for understanding TGF-beta pathway in glioblastoma cancer.

Related Dissertations

  1. Quantiative Proteomics of Liver Immortalized Cell Line Chang Liver and Liver Cancer Cell Line HepG2,R735.7
  2. Human glioblastoma resveratrol metabolites and metabolizing enzymes expression analysis,R739.4
  3. Diazoxide post-processing in the rat heart mitochondria comparative proteomics research,R965
  4. Study of Diffusion Tensor Imaging and Proton-related Spectral in Intracranial Tumor,R445.2
  5. Banana Leaves of Low Temperature Stress SSH Library Construction and Differential Protein Study,S668.1
  6. The Impact of Invasiveness for Human Original Malignant Glioma Cells by the Changes of the Calcium and Magnesium Ions Environment in Vitro,R739.4
  7. Differential Proteomics Analysis of the Zaosu Pear and Its Red Bud Mutation,S661.2
  8. Study on the Genetic Susceptibility between KDR and Gliomas in Chinese Population,R739.4
  9. Effects of Methylpredisolone on the MGMT Expression and the Radiation Sensitivity in the Synchronous Radiotherapy with Temozolomide in Vitro in Glioblastoma,R739.41
  10. The Correlationship between the Distribution of CD133-positive Cells and the Proliferation of Microvessels in Glioblastoma Multiforme,R739.4
  11. Effects of Salusin on Vascular Smooth Muscle Cells Proliferation and Proteomic Profiling of Heart Treated with Salusinβ,R54
  12. Realgar Intervenes Invivo Bcl-2 and Bax Expression in the Original Generation of Human Brain Glioblastoma Cells,R285
  13. Experimental study of the spleen Yin deficiency syndrome motilin in rats , pepsin , aquaporin- 4 and proteomic changes,R241
  14. Proteomic Analysis of Sut Melanocytes in Response to xCT Deficiency,R341
  15. Study Cerebrospinal Fluid Proteomic in Rats after Acute Spinal Cord Injury by SELDI-TOF-MS Technique,R651.2
  16. Abnormal Protein Expression and Modification in the Spinal Cord with ALS,R744.8
  17. Proteomic Study of Hippocampus and Cerebral Cortex in the Rats Model of Temporal Lobe Epilepsy,R742.1
  18. Differential Protein Expression in Serum of Cervical Carcinoma and Preliminary Proteomics Study of HeLa Cells after Radiation Therapy,R737.33
  19. Quantitative Proteomic Study of Laser Capture Microdissection Purified Normal Human Bronchial Epithelia and Human Lung Squamous Carcinoma,R734.2
  20. Heat shock protein 60 in the left colon and right colon tissue Differential Expression,R735.35
  21. The Pharmacokinetics Study of Ivabradine Hydrochloride Tablets in Body,R96

CLC: > Medicine, health > Oncology > Nervous system tumors
© 2012 www.DissertationTopic.Net  Mobile