Dissertation > Excellent graduate degree dissertation topics show

Study on the Correlation between CD4+CD25+Foxp3+ Regulatory T Cells and TGF-β1、IL-10 in Peripheral Blood of Esophageal Carcinoma in Hazak

Author: ZhangJian
Tutor: HuangYanChun
School: Xinjiang Medical University
Course: Clinical Laboratory Science
Keywords: Hazak esophageal cancer regulatory T cell TGF-β1 IL-10 flow cytometry
CLC: R735.1
Type: Master's thesis
Year: 2011
Downloads: 46
Quote: 0
Read: Download Dissertation

Abstract


Objective:To explore the correlation between CD4+ CD25+ Foxp3+ regulatory T cells and TGF-(3,.1L-10 through testing the change of CD4+ CD25+ Foxp3+ regulatory T cells,TGF-β1 and IL-10 in peripheral blood of esophageal cancer patients in Hazak according to different TNM stages. Methods:The study was carried out in a total of 47 Hazak patients with esophageal cancer diagnosed by Xinjiang Medical University affiliated Tumor Hospital from January to November in 2010.All patients were newly diagnosed and none had received any form of anti-cancar therapy before collection of their peripheral blood for test.30 healthy Hazak volunteer were included in this study as the control group.1. Flow cytometry was used to analyze the proportion of CD4+ CD25+ Foxp3+ regulatory T cells in CD4+ T cells in peripheral blood mononuclear cells (PBMCs) of 47 Hazak patients with esophageal cancer and 30 healthy peoples.2. enzyme-linked immunosorbent Assay (ELISA) was used to detect the level of TGF-β1、IL-10 in 47 Hazak patients with esophageal cancer and 30 healthy peoples. Results:1. the proportion of Treg cells, the expression level of TGF-β1 and IL-10 in the case group were obviously higher than that of the control group, the difference has statistics significance (P< 0.05).2. compared with control, there were not statistically significant difference of the proportion of Treg cells, the expression level of TGF-β1 and IL-10 in phase of TNM stage (Ⅰ～Ⅱ) and without lymph node metastasis (P>0.05), there were statistically significance of the proportion of Treg cells, the expression level of TGF-β1 and IL-10 in phase of TNM stage (Ⅲ～Ⅳ) and lymph node metastasis (P<0.05); the difference of the proportion of Treg cells, the expression level of TGF-β1 and IL-10 between the stage of TNM(Ⅰ～Ⅱ) and (Ⅲ～Ⅳ) was significant (P<0.05).3.the expression level of TGF-β1、IL-10 was significantly correlated with the proportion of CD4+ CD25+ Foxp3+ regulatory T cells (P<0.01).Conclusion:1. the proportion of Treg cells in peripheral blood of the Hazak esophageal patients significantly increased, it probably had certain value in judging disease stage of esophageal cancer in Hazak by detecting the proportion of Treg.2. the expression level of TGF-β1、IL-10 were obviously increased,it was probably that TGF-β1 and IL-10 could inhibit the body’s anti-tumour immune responses.3. the expression level of TGF-β1, IL-10 were significantly related to Treg cells, and it may be involved in the immune negative adjustment mechanism of body’s.

Related Dissertations

  1. Study the Protective Value of Spect Lung Perfusion to Lung Function in Esophageal Cancer Patients Treated with Radiotherapy,R735.1
  2. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  3. Noble Dendrobium Polysaccharides Attenuate Learning and Memory Deficits Induced by Lipopolysaccharide and Its Mechanisms in Rats,R285.5
  4. Effect of Grape Seed Procyanidins on the Blood Pressure in Renovascular Hypertensive Rats and Its Mechanism,R544.1
  5. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  6. Diagnosis Value of Cytokine Levels and TB-Ab in Serum in Children Tuberculosis and Latent Tuberculosis Infection,R725.1
  7. Protein Expressions of EGFR、K-ras、P53 and Ki-67 in Colorectal Cancer, Gastric Cancer and Esophageal Cancer,R735
  8. Relationship between Expression of COX-2 and Cell Cycle Regulatory Proteins in Patients with Esophageal Squamous Cell Carcinoma,R735.1
  9. Osthol on bleomycin- induced pulmonary fibrosis in rats and its mechanism of,R563.9
  10. Sonic Hedgehog pathway and Caveolin-1 protein expression in esophageal cancer,R735.1
  11. The Dynamic Changes of Serum Cytokines in Paraquat Poisoning Rats and the Artesunate Intervention Studies,R595.4
  12. Anti-tumor Effects of Lupeol at Different Regimens and Its Role in Immune Systems,R730.5
  13. Protection of Probiotics from Damage to Neonatal Rat Model of Necrotizing Enterocolitis,R722.1
  14. Research on the Swimming and Cordyceps’ Effect on the Expression of Splenic CD4~+ CD25~+ Treg of Mice,G804.2
  15. Risk Factors of Stroke-associated Infection and Its Correlation with Stress Response and Cell Immunity,R743.3
  16. The Therapeutic Role of Adenovirus Vector Mediated Rat Interleukin 10 (rIL-10) Gene to Streptozotocin Induced Type 1 Diabetes Mellitus of KM Mice,R587.1
  17. Expression of Transcriptional Corepressor C-terminal Binding Protein1 (CtBP1) and p16 in Esophageal Cancer,R735.1
  18. The Expression of IL-10, TNF-α in Prostate Hyperplasia and Prostate Cancer Tissue and Its Significance,R737.25
  19. Study of Dendriti Cell Treatment Combined with Chemotherapy for Late Gastric Cancer,R735.2
  20. Clinical Comparative Study of Postoperative Early Enteral Nutrition and Total Parenteral Nutrition in Old Esophageal Cancer Patients Undergoing Esophagectomy and Esophageal Replacement with Gastric Tube,R735.1
  21. The Effect of Low-intensity Pulsed Ultrasound on Cell Proliferation and the mRNA Expression of Extracellular Matrix of SD Rat Precartilagious Stem Cells,R329

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Esophageal tumors
© 2012 www.DissertationTopic.Net  Mobile