Dissertation > Excellent graduate degree dissertation topics show

The Clinical Research of Monitoring Minimal Residual Disease in Leukemia Patients by Fluorescence in Situ Hybridization

Author: ZhangZhengZuo
Tutor: QuJianHua
School: Xinjiang Medical University
Course: Internal Medicine
Keywords: fluorescence in situ hybridization(FISH) minimal residual disease(MRD) graft versus host disease(GVHD)
CLC: R733.7
Type: Master's thesis
Year: 2011
Downloads: 32
Quote: 0
Read: Download Dissertation


Objective:To investigate the effect of dynamic follow-up minimal residual disease (MRD) of leukemia patients by fluorescence in situ hybridization (FISH),assess the treatment by MRD and predict the occurrence of GVHD. Methods:85 specimens from 44 leukemia patients after chemotherapy or allogeneic stem cell transplantation were studied by FISH and conventional cytogenetic analysis. Results:1.FISH analysis showed that 15(17.6%) samples were positive. Conventional cytogenetic analysis showed that 11(12.9%)samples were positive. The two methods have no significant difference;2. MRD-positive patients recovered after the appropriate treatment. The FISH results after transplantation have no difference in statistics (P>0.05);3.The result of FISH showed that the percentage of positive cells in the treatment group were lower than the before treatment group (P<0.01), but still can not believe that the drug treatment group and the hematopoietic stem cells transplant group have difference in statistics (P>0.01); 4. The incidence of acute graft-versus-host disease (GVHD) has no correlation with the results of FISH of 1 month after transplantation (P>0.05). Conclusion:1. FISH and conventional chromosome analysis has no significant difference in detection rate, but faster, more sensitive, more suitable for clinical use;2.Using FISH monitoring MRD is important to evaluate the status of patients and guide corresponding treatment;3.FISH can reflect the treatment effect accurately;4.MRD still can not accurately predict the occurrence of GVHD.

Related Dissertations

  1. Clonal Origin of Multifocal Clear Cell Renal Cellcarcinoma Studied by Fluorescence in Situ Hybridization,R737.11
  2. The Study on Clinical Using of FISH and Cytology in Diagnosis and Surveillance of Recurrence in UC,R737.1
  3. Clinical Analysing of Cases Regarding Unrelated Umbilical Cord Blood Transplantation (UCBT) for the Treatment of Hemotologic Disease,R725.5
  4. Bacterial Population in Sulfide Nickel and Copper Ore Bioleaching System and Its Bioleaching Efficiency,TF18
  5. A Preliminary Study of the Effect of G-CSF on MDSCs in Bone Marrow and Peripheral Blood,R329
  6. Foodborne Listeria Monocytogenes: Peptide Nucleic Acid Fluorescence in Situ Hybridization and Antigenicity of Its Surface Protein LMO1847,Q93-3
  7. Study on Telomerase RNA Component Expression and HPV Infection in Cervical Intraepithelial Neoplasia,R737.33
  8. Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer,R737.9
  9. The Relationship between Segmented Filamentous Bacterias (SFB) and Host Immunity System and Preliminary Culture,S852.4
  10. The Research of Prenatal Diagnostic Program of Chromosomal Aneuploidy,R714.5
  11. Screening of Linkage Group -Specific BAC Clones in Chinese Cabbage and FISH Analysis in Cabbage-Chinese Cabbage Monosomic Alien Addition Lines 1# and 4#,S634.1
  12. The Clinical Application of Fluorescence in Situ Hybridization in Detecting the Chromosomal Abnormality of Spontaneous Miscarriage Tissue,R446.8
  13. Study on the Expression and Clinical Significance of hTERC Oncogene in Cervical Exuviation Cells,R737.33
  14. FISH Analysis in Cabbage-Chinese Cabbage Allotriploid Hybrid and Chinese Cabbage Alien Addition Lines,S634.1
  15. Isolation and Evolutional Mechanism Analysis of One Triticeae St-Genome Pericentromeric Retrotransposon,S512.1
  16. Investigate the Distribution Feature of bc16 Gene Isolation of DLBCL in Guangxi,R733.1
  17. The MDS chromosome karyotype analysis and MM cytogenetic detection method comparison,R446.9
  18. Application of Fluorescence in Situ Hybridization (FISH) of Placental Trophoblast Cells to Detect the Number of Chromosome,R714.5
  19. Study on the Diversity of Ammonia Oxidizing Bacteria in Municipal Wastewater Treatment Plants,X703
  20. Volatile Fatty Acids Production from Sewage Sludge by Anaerobic Countercurrent Fermentation,X703

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile