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The Expression of IgA in EIN and Esophageal Cancer

Author: WangJiHong
Tutor: SuMin
School: Shantou University
Course: Pathology and Pathophysiology
Keywords: Esophageal carcinoma EIN IgA Ki-67
CLC: R735.1
Type: Master's thesis
Year: 2011
Downloads: 6
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Abstract

Background and ObjectiveTraditionally IgA is only produced and secreted by plasma cells. However, resently severalresearches have shown that cancer cell can express IgA, such as human breast carcinoma cellline, colon carcinoma cell line, human gastric carcinoma cell line et al. And research in vitrosuggested that IgA derived from epithelium cancer cell line could promote the growth of cancercells. It’s still not clear that if the esophageal cancer cell can express IgA mRNA and protein.As one of the top six malignant tumors, more than 50% new cases of esophageal cancer inthe world have occurred in China. It has become a health-harm factor of our country. Presently,the research about the expression of IgA in esophageal cancer has been limited to plasma cells incancer stroma. But whether the esophageal cancer parenchymatous tissue can express IgA hasnot been reported. In this article, we use Immunofluorescence Cell Staining，IHC and NestedRT-PCR to test expression of IgA protein and mRNA in esophageal cancer cell line EC109 andnormal esophageal tissue, observe the expression of IgA in normal, inflammatory ,EIN andcancerous tissues in Chao Shan high incidence area of esophageal cancer and compare thedifferences of the expression to explore the relation between the IgA expression andinflammation or the proliferation of esophageal carcinoma.Materials and MethodsMaterials148 cases of specimen were totally collected , including 81 cases of matched esophagealcancerous tissues and normal tissues and 35 cases of EIN tissues (collected from Tumor Hospitalof Shantou University Medical College since 2008) and 14 cases of gastroscope biopsy normalesophageal tissue and 18 cases of gastroscope biopsy inflammation specimen (collected fromjieyang People’s Hospital since 2009). Tumor histology type was squamous cancer thoroughly.According to pathological classification, there were 23 cases of gradeⅠ, 46 cases of gradeⅡ,and 12 cases of gradeⅢ. Of EIN tissues, 20 cases of low grade EIN (15 of EINⅠand 5 ofEINⅡ) and 15 of high grade EIN (EINⅢ) were included. Of gastroscope biopsy inflammation specimen, 10 cases of mild degree and 6 cases of moderate degree and 2 cases of severe degreeinflammation were contained.Methods1. Immunofluorescence Cell Staining : To test the expression of IgA protein in EC109 cellline.2. Nested RT-PCR : To detect IgA mRNA expression in EC109 cell line and normalesophageal tissue obtained by microdissection .3. HE stain : Tumor histology type，differantiation and inflammation were observed by lightmicroscope after HE stain.4. Immunohistochemistry : The Envision Labelled Peroxidase System two steps methodwas performed in the study to test the expression of IgA in the normal, inflammatory, EIN, andcancerous esophageal tissues and the expression of Ki-67 in cancerous esophageal tissues .5. Image analysis and results assessing of IHC : The results of IHC was analyzed by LeicaIM50 picture collecting system and Image-Pro Plus 6.0 picture analyzing system.6. Statistical analysis : SPSS13.0 statistical software was used for statistical analysis. Theanalysis methods of Nonparametric Test, One-Way ANOVA and Spearman Rank Correlationwere employed.Results1. IgA protein existed in normal and inflammatory esophageal tissue.With the increasing ofinflammatory degree, the expression of IgA in esophageal epithelium had a tendency ofincreasing, they also have a tendency of positive correlation, but the difference was notstatistically significant (P＞0.05).2. We could see the expression of IgA in EIN tissues, with increasing degree of cell atypia, IgAexpression tends to increase, they had a tendency of positive correlation, but the difference wasnot statistically significant (P＞0.05),3. IgA protein and mRN A expression could be detected in EC109 cell line, IgA mRNA couldalso be tested in normal esophageal tissue.4. IgA protein expressed in cancerous esophageal tissue, mostly in the cytoplasma of cancercells. The expression of IgA in cancerous tissues was lower than in high degree EIN and normal tissues obviously (P＜0.05). In different expression degree groups of IgA , the Mean LI of Ki-67was (－): 0.426±0.120, (+): 0.435±0.131，(++): 0.546±0.148 seperately. The disparity of LI indifferent expression groups of IgA was stastically significant (P＜0.05) and the expression ofIgA had a positive correlation with LI (rs =0.223，P＜0.05).Conclusions1. IgA protein and mRNA expression can be identified in normal esophageal epithelium.2. There is a tendency of positive correlation between IgA expression and the degree ofinflammation or EIN.3. It’s firstly discovered that IgA protein and mRNA express in esophageal cancer cells. IgAprotein can also be tested in esophageal cancerous tissue and its expression have a positivecorrelation with proliferation of esophageal carcinoma. It needs more researches whether the lowlevel expression of IgA in esophageal cancerous tissue have association with structuralabnomalities of IgA.

The Expression of IgA in EIN and Esophageal Cancer

Abstract

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