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Aim To discuss targeting macrophage migration inhibitory factor (Macrophage migration inhibitory factor, MIF) siRNA on BALB / c mice liver metastases of colorectal cancer and its possible mechanism. Method 1 . Obtained solid tumors . 2 cecal tumor block made ??hernia was established by orthotopic mouse model of colorectal cancer liver metastases : According to our previous reports made ??cecal hernia was established by orthotopic tumor block colorectal cancer metastasis model in mice . Postoperative 1w, abdominal subcutaneous mouse model can reach a size of about bean nodules . Postoperative 4w, visible 1.8cm * 1.5cm * 1.4cm sized tumor block , row histopathological examination showed liver metastases , modeling success. 3 transfected with siRNA targeting MIF intervention : the successful modeling mice were randomly divided into three groups. In situ tumors were injected twice a week for the same volume of targeting the MIF siRNA (MIF siRNA, 0.15ng/kg), NS-siRNA (0.15ng/kg) and normal saline (NS, 0.15ng/kg), treatment 4 weeks. 3 days after the end of treatment , the mice were sacrificed . 4 consecutive liver sections, HE staining of liver metastases of colorectal cancer in mice in each group . 5.ELISA serum MIF and vascular endothelial growth factor (VEGF) concentration . 6 Immunohistochemical detection of liver metastases microvessel density . Results MIF siRNA group , NS-siRNAR group and NS group of colorectal liver metastasis rates were 10% , 60% and 70% (x ~ 2 = 8.30, p lt; 0.05), serum MIF were (20 ± 4) pg / ml, (72 ± 8) pg / ml and (78 ± 7) pg / ml, (p lt; 0.05); mouse serum VEGF were (20 ± 4) pg / ml, (77 ± 9 ) pg / ml and (77 ± 10) pg / ml, (p lt; 0.05); liver metastases MVD were (19 ± 3), (29 ± 6) and (35 ± 7), (p lt; 0.05 ) . Conclusion The siRNA targeting MIF mice reduced the incidence of colorectal cancer liver metastases , which may be inhibited by siRNA targeting MIF MIF expression, reduced expression of VEGF , reducing the MVD.
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