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Study on ERCC1 Expression and Single Nucleotide Polymorphism Related to Sensitivity of Squamous-cell Lung Carcinomas to Cisplatin in Vitro

Author: ZhouWeiHua
Tutor: DengZuoYun
School: Nanchang University
Course: Oncology
Keywords: Cisplatin Excision repair cross-complementing gene 1 Single nucleotide polymorphisms Adenosine triphosphate - tumor cell susceptibility testing Polymerase chain reaction - restriction fragment length polymorphism
CLC: R734.2
Type: Master's thesis
Year: 2009
Downloads: 59
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Abstract


Objective: To investigate lung squamous cell carcinoma (Squamous-cell lung carcinoma) tissue excision repair cross-complementing gene 1 (excision repair cross completion gene ERCC1) expression and ERCC1 codon 118 single nucleotide polymorphisms (single nucleotide polymorphism, SNP) relationship of cisplatin in vitro susceptibility, provide important theoretical basis for the guidance of the individual treatment of cisplatin used in lung cancer. Methods: 1, fresh specimens collected 42 cases of surgical resection of lung squamous cell carcinoma patients immediately with adenosine triphosphate - tumor cell susceptibility testing (ATP-tumor chemosensitivity assay, ATP-TCA) cisplatin in vitro susceptibility testing. 2, streptomycin avidin - peroxidase method (SP) of the paraffin-embedded specimens of 42 cases of lung squamous cell carcinoma patients ERCC1 protein expression was detected by immunohistochemistry. 3, using the polymerase chain reaction - restriction fragment length polymorphism (polymerase chain reaction-restrictive fragment length polymorphism, PCR-RFLP) technology specimens ERCC1 codon 118 (C → T) gene polymorphism. Finally, comparative analysis of ERCC1 protein expression of ERCC1 codon 118 (C → T) gene polymorphism with cisplatin in vitro susceptibility. Results: 1,42 cases of lung squamous cell carcinoma, and cisplatin in vitro effective for 22 cases (52.4%), in vitro invalid in 20 cases (47.6%). ERCC1 protein expression group 2, 22 patients in the cisplatin in vitro effective in 8 cases (36.4%), 20 patients with ERCC1-negative expression group, cisplatin in vitro effective in 14 cases (70.0%) of ERCC1 positive expression sensitive to cisplatin significantly lower than those with negative expression (P lt; 0.05). 3,17 cases (40.5%) of the ERCC1 codon 118 AAC / AAC homozygous (C / C type), 15 patients (35.7%) for the AAT / AAT homozygous (T / T-type), 10 patients (23.8%) AAC / AAT heterozygous (C / T); T / TC / T in patients with cisplatin, an effective rate of 68.0% (17/25), C / C-type effective rate was 29.4% (5/17), with T base T / TC / T in patients with than T base-free C / C patients on cisplatin chemotherapy respond better (P lt; 0.05). 4, ERCC1 protein expression of ERCC1 codon 118 (C → T) polymorphism contact exists between the experimental data show that the T base-containing T / TC / T in patients with ERCC1-positive expression rate was significantly lower than the C / C genotype (P lt; 0.05). Conclusion: The positive expression of ERCC1 may be associated with lung squamous cell carcinoma associated cisplatin resistance. ERCC1 codon 118 T base T / TC / T-type patients than T base-free C / C patients with a better response to chemotherapy of cisplatin. 3, T / TC / T genotype in patients with ERCC1-positive expression rate was significantly lower than the C / C genotype, which may explain the T / TC / T in patients with cisplatin chemotherapy than the C / C genotype patients respond better reasons . 4, ERCC1 protein expression of ERCC1 codon 118 (C → T) close links that may exist between single nucleotide polymorphisms and cisplatin chemotherapy response, ERCC1 cisplatin chemotherapy for lung squamous cell carcinoma patients is expected to become the predictors.

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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