|
Objective: alginate microcapsules coated with immune isolation effect of allogeneic Schwann cells, and transplantation for the treatment of spinal cord injury after spinal cord injury was observed by immunohistochemistry microencapsulated nerve tissue cell transplantation c-fos expression changes. Methods: under sterile conditions in rat sciatic nerves made of tissue cell suspension, 1.5% sodium alginate solution mixed with low-speed centrifugation and injected into the 20mmol / L barium chloride solution made of microencapsulated sciatic nerve tissue cells . 95 adult Sprague-Dawley (SD) rats were randomly divided into four groups: the microcapsules group (A), the cell suspension group (B), simple injury group (C group) and sham operation group (D group): sham operation group 5; 30 rats in each other three groups of rats after T8 right side of the hemisection, were implanted into the cells of the the adsorbed gelatin sponge microencapsulated sciatic nerve tissue, the the gelatin sponge adsorption sciatic nerve tissue cell suspension and gelatin sponge, postoperative 6h, 12h, 24h, 3d, 7d, 14d, n = 5 rats, 4% paraformaldehyde attentive - liter aortic perfusion removed after about two segments of the damage zone caudal spinal cord and corresponding parts of the spinal ganglion, the D rats take the appropriate parts of the spinal cord and dorsal root ganglia. Paraffin sections underwent immunohistochemical staining and image analysis technique to observe c-fos in the spinal dorsal horn and anterior horn and dorsal root ganglia (dorsal root ganglion, DRG) content changes. Results: 6h after spinal cord hemisection, c-fos positive neurons increased, while positive cells accumulated optical density (IOD) value also increased c-Fos protein can be seen in the increased expression of gray matter, anterior horn and posterior horn positive cells but mainly in the posterior horn. 12h after injury began to decline, and then appeared to rise at 24h, compared with sham group (4.05 ± 0.38). Single loss group c-fos positive cells in the 3d started to decrease postoperative 7D, 14d a single loss group with the sham group, no significant (P lt; 0.05). The microencapsulated of c-fos expression in 6h, 12h, 24h, 3d are significantly lower than the single-loss group and the cell suspension group, the difference was statistically significant (P lt; 0.05). Cell group at 6h and 12h with a single loss group was statistically significant (P lt; 0.05) 7d and 14d in each group was not statistically significant (P gt; 0.05) Conclusion: (1) microencapsulated cell transplantation can reduce spinal c-fos expression, has a protective effect on the nerves in the spinal cord hemisection yuan. (2) of microencapsulated Schwann cell transplantation may be the expression of c-fos down to a certain extent reduce secondary injury of the spinal cord.
|