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Mulberry, commonly used as a traditional Chinese medicine, is currently on its composition and quality control aspects of the research are not too deep , the lack of a more comprehensive qualitative and quantitative methods . So the use of new separation and analysis of its chemical composition and quality control study has important significance. Firstly, high-efficiency and high-speed countercurrent chromatography preparative liquid separation technology for the chemical composition of mulberry were isolated and purified , isolated by five high-purity compounds , and through MS and NMR structures were confirmed , namely mulberroside A, mulberroside F, trans- oxidation resveratrol, trans-resveratrol and Sangxin Su M, where F is the first time mulberroside isolated from the medicine . On this basis , for the first time to establish an accurate , reliable, reproducible, and can be determined simultaneously one of the five components by HPLC method , combined with the similarity analysis , principal component analysis and cluster analysis, chemometrics methods 25 batches of samples from different areas mulberry fingerprints were analyzed and found that the sample and the similarity between the chemical composition varied widely , indicating that the quality of each batch of mulberry is not the same . From the principal component analysis and cluster analysis diagram figure also found in the sample can not be classified according to origin , indicating little mulberry quality with the origin , perhaps with mulberry harvest time , processing methods and so on. This study of mulberry ingredients and their fingerprint analysis for quality control of mulberry study provides experimental evidence for the effective development and take full advantage of mulberry medicine has important reference value.
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