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The Effects of Different Traditional Chinese Medicine Therapy on the Expression of GSK-3β in Hippocampal Region of the AD Mice Model

Author: ChenLie
Tutor: XueLi
School: Central South University
Course: Traditional Chinese Medicine
Keywords: Bushen Huoxue method alzheimer’s disease Yizhi Jian nao granules Morris water maze GSK-3β
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 23
Quote: 0
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Abstract


Objective:To investigate the effects of Bushen Huoxue(Yizhi Jiannao granule) method、Bushen(Decoction of Rehmanniae granule) method and Huoxue (Xuefu Zhuyu granule) method decoction’s on Alzheimer’s disease model rats’ learn and memory ability and the expression of GSK-3βin hippocampus, this work discusses partially the mechanism and preponderance of traditional Bushen Huoxue method to treat Alzheimer’s disease.Methods:60 SD male rats were randomly divided into six groups by weight, normal group, sham controls group, model group, Bushen Huoxue group, Bushen group, Huoxue group, and various groups of 10 only. The last Four groups injects beta amyloid protein 25-35 (Aβ25-35) in bilateral hippocampal CA1 region, sham controls group injection steam water, normal group received no special treatment. The Bushen Huoxue group、Bushen group and Huoxue group are gavaged separately Yizhi Jiannao granule、Decoction of Rehmanniae granule and Xuefu Zhuyu granule, the other theree group are gavaged such equal dose double-distilled water. And after operation 21 days and Chinese medicine therapy eight weeks, carry out MWM(morris water maze test) behavior detection.Last,the hippocampal of rats were taken out to examine the expression of GSK-3βby semi-quantitative RT-PCR and immunohistochemistry SABC method.Results:1. Behavioral results:compared with other treatment group, the Bushen Huoxue group can effectively improve AD of rats’ ability to learn and remember.2. RT-PCR and immunohistochemical result:compared with other treatment group, the Bushen Huoxue group can effectively reduce the level of GSK-3βin the AD mice model.Conclusion:1. Bilateral hippocampal injection by Aβ25-35 can build successful AD model.2. The Bushen Huoxue method can effectively improve learn and memory ability in the AD mice model, superior to Bushen group or Huoxue group.3. After the Bushen Huoxue method therapy, the level of GSK-3pin the AD mice model were decreased, by inhibiting through GSK-3βactivity can reduce the phosphorylated proteins abnormal high level of Tau beta, prevent Aβin neurons, superior to Bushen group or Huoxue group.

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