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Study on Extraction, Purification and Activity of Polysaccharide in Saccharum Alhagi

Author: WuJing
Tutor: ChangJunMin
School: Xinjiang Medical University
Course: Pharmaceutical Analysis
Keywords: Thorn polysaccharide Extraction Activity Amino acids
CLC: R284.1
Type: Master's thesis
Year: 2011
Downloads: 62
Quote: 1
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Abstract


Objective: To investigate stab sugar optimum extraction conditions of polysaccharide and polysaccharide sugars thorns were isolated; study the nature of polysaccharides stab sugar, monosaccharide composition and antioxidant activity; compounds obtained and preliminary structural analysis. Method: 1. Using orthogonal test method to determine the stab sugar polysaccharide optimum extraction conditions. (2) using DEAE-52 cellulose and Sephadex G-150 gel column chromatography method under optimum conditions stab sugar extracted polysaccharides were isolated and purified. 3 of thorns polysaccharide cellulose column separated by a stream of physical and chemical properties of S1 and by TLC on its preliminary analysis of monosaccharide composition. 4 by UV, IR and NMR spectra of the compound obtained preliminary structural analysis. 5 of stab sugar polysaccharide antioxidant activity. 6 using fluorescence spectrometry stab sugar amino acids. Results: 1. Determine stab sugar polysaccharides optimum conditions: at 70 ℃ with nine times the amount of water, reflux extraction 1.5h, concentration of alcohol precipitation is 80%. (2) using optimal extraction Extraction of crude polysaccharides obtained during the extraction process and the two compounds, polysaccharides through DEAE-52 cellulose column separated six fractions S1, S2, S3, Y1, Y2, Y3. 3 initially identified monosaccharide composition of the main Portuguese sugar. 4 through structural analysis of the two compounds were obtained D-Glu-1α → 2β-D-Fru, D-Glu-1β → 6-D-Glu-1α-2β-D-Fru. 5 stab sugar polysaccharide by the antioxidant activity of drawn: polysaccharide hydroxyl radical S1 has better efficacy, clearance rate increases with the concentration; and its absorbance at 700nm with concentration increases, indicating that there is a strong reducing power, is a good antioxidant .6. fluorimetric determination of amino average content of 0.9163mg · g-1. Conclusions: 1. Verify the results obtained through the verification test and orthogonal experiment were similar, indicating that the process is basically stable and feasible. (2) using DEAE-52 cellulose column achieve the effect of separation, come to different fractions. 3.TLC method to determine the initial composition of the primary monosaccharide glucose. 4 obtained by the structural analysis of the compound obtained disaccharides and trisaccharides structures. 5 thorn polysaccharide on free radical scavenging and antioxidant effects can intimidate radical oxidative damage, thereby protecting the prevention of certain diseases cell function is important. 6 fluorescence method is simple, fast and accurate determination of the highest sensitivity, detection limit of 9.70 × 10-7 mol · L-1, is the determination of amino acids in natural medicine a better way.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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