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Purpose :This research is through the reproduction of the model that young mouse lung often inadequate , on this basis through iv induced replication lung often inadequate mouse pneumonia model. by the composite model of successful replication to look to the wind scattered to the protection, and mouse and organizations of different AQP4 expression and access to examine them from the water to the lungs often inadequate mouse protection mechanisms, part of the possible for to the wind scattered the lung disease prevention often inadequate to provide an experimental basis.Methods:1.The smoke make mouse lung often inadequate model, by observation mouse, and the general index and mouse and the organization’s show pathological changes to verify that the model.2.Use the replication mouse lung often inadequate model , then use a concentration of influenza viruses liquefier drop of a viral infection with the mouse preparation of acute pneumonia model, through breathing, coat, catering and weight and change the appearance of the lungs of the organization, lung organization is under the pioneer and lung index for measuring the lungs often inadequate pneumonia model.3.The use of chemical technology detection normal immune organizations, groups and to the model of the wind high, medium and low dose of the lungs of the mouse every group AQP4 whites. a virus infections in rodents every group AQP4 express take to change the situation, the wind scattered to the prevention of lung disease often inadequate to provide support.Results:1.Normal group maintained a good state of the basic model for the stimulation of breathing, eating coat, and stimulating change. the ten days later, the model of the lungs of the organization of pathological and appearance have a change. the two groups and mouse with a significant gender difference index (p<0.05).2.Model set for the mouse to stimulate and influenza virus breathing, coat, catering and weight, etc. organization of pathological changes from the lungs, drowning mouse normal group alveolus size is, the integrity of the epithelial, such as secretions, it is, to see an inflammatory cells alveolus wall not thickend ; model group alveolus alveolus mouse, and organizational structure of normal alveolus, alveolus interval thickend, swelling, a large number of inflammatory cells. infiltration alveolus the capillaries are spreading red.3.Lung index for measuring results : model set for the mouse to stimulate and influenza virus lungs were congested his lungs and a dirty obvious, an increase in lung index increased. the normal groups and groups are models are remarkable differences(p<0.05).4.From time to time some of the two groups live mouse only a few more observations on the two groups of seven days, rodents to live normal group of all living. the model group of seven days in the death of four, on the fifth day to death, to be a significant gender difference groups (p<0.05).5.Away the Yupingfeng, middle and lower the general condition is improving the mouse, reduce lung infection, and lungs in the reduction in the wind scattered to the high dose of action is preferable to the wind scattered in the wind scattered groups and to lower the difference groups, a statistical significance(p<0.05); to the wind scattered in the group and to the wind scattered the relatively low, and no statistical significance(p>0.05).6.Lung organization AQP4 protein express situation normal set of the albumen detected AQP4 express ; model group to group and organization, AQP4 express clearly, that diversity has a statistical significance(p<0.05); to the wind scattered high doses, to the wind scattered groups of the group, to the wind scattered the group of low AQP4 express below the normal group with statistics,the difference in meaning. to the wind scattered the group and to the wind scattered the group is low and no statistical significance (p>0.05).Conclusion:1.Model for the stimulation of mouse were breathing, coat, catering to the index changes, lung, lung look and organization of the lungs of the organization is under the pioneer in the evaluation model provides reliable objective basis. through the excitement to reproduce the success of the lungs often inadequate mouse model, the method is simple.2.Model set for the mouse to stimulate and influenza virus breathing, coat, catering and weight and change the appearance of the lungs of the organization, lung organization is under the pioneer and lung index for measuring lung often inadequate pneumonia model provides the basis of objective. influenza virus to adapt to a single drop of a lung infection lung often inadequate mouse can reproduce the success of the lungs often inadequate mouse pneumonia model3.Away the Yupingfeng to lung infection often inadequate mouse influenza viruses have the protective function, and motivate AQP4 in protein level of expression, in order to improve alveolus capillaries are, the protection of water between the lungs of the organization.
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