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ObjectiveMorinda is Dry root of Rubiaceae,It Is a famous one of the four southern medicine。Only contained in the "Shen Nong’s Herbal Classic", as a top grade, followed by ancient herbal medicine were recorded.Morinda sweet, acrid, of tepid, functional kidney yang, strengthens bones and tendons, rheumatism.Commonly used in clinical impotence and nocturnal emission, cold uterus infertility, Fengshibitong, irregular menstruation, Shaofu Leng Tong, bones and muscles atrophy soft embolism.Morinda officinalis in the widely used prescription medicine, is one of the important varieties of Chinese herbal medicines.Pharmacopoeia 2010 edition contains four processing Morinda species, Morinda officinalis in addition to raw materials, the rest had no clear quality standards, no precise processing parameters, a rule of thumb operation, different operators and manufacturers of processed products of different quality All the same, affecting the quality of the stability of the Pieces.This topic aims to establish a scientific and feasible Morinda processing technology specifications, determine the processing technology to develop quality standards for Pieces of Morinda officinalis, Morinda analytical processing principles.Materials and methodsMaterialsRadix Morinda officinalis purchased from Guangdong Deqing growing base of Chinese medicine by the Liaoning University of Traditional Chinese Medicine Identification, Professor Department of clouds are identified as the beginning of the Rubiaceae genus Morinda officinalis Morinda officinalis How.’s dried root.Methods1. Nice sugar and total polysaccharides in the index, salt content, moistening and steaming time as the inspection time factor, the orthogonal experimental design optimization of processing technology of salt Sunburn officinalis.2. Nice sugar and licorice with ammonium as an indicator, licorice water, moistening the time factor and the boiling fire was investigated by orthogonal design optimization Sunburn Morinda licorice water processing technology.3. Application of column chromatography, preparative TLC, preparative liquid chromatography herbs from Morinda isolated 11 compounds, the use of UV, IR, NMR and MS spectra and other means of identification of the compounds.4. Application of liquid chromatography before and after processing compared Morinda anthraquinone chemical composition changes.5. The mice fed with methods used, and organ weight changes in mice, comparing before and after processing Morinda tonic effect; hypoxia by measuring the time to compare before and after processing Morinda anti-stress effect of changes .6.Nice sugar as an indicator to develop processed products of Morinda officinalis quality standards. Of ten lots of different places Morinda species different processing impurities, moisture, ash, extract, etc. were determined to develop officinalis and its processed products of quality standards.Results:1. Sunburn Morinda determine the best salt processing technology, that is, 100 g officinalis 2% salt solution in 100 mL after the steam moistening 5h 1h, cool and dry. Salt consumption 100:2 (herbs: salt).2. Sunburn Morinda determine the best licorice water processing technology, that is, 100 g to 1.5 times the amount of Morinda officinalis (150 mL) aqueous moistening licorice 3 h, high heat until boiling liquorice water exhaustion. Licorice amount of 100:6 (Morinda: Licorice).3. Application of column chromatography, preparative TLC, preparative liquid chromatography herbs from Morinda isolated 11 compounds, the use of UV, IR, NMR and MS spectra and other means of identification of the compounds.5. Fed mice by measuring the liver, kidney and spleen weight, that the salt water Sunburn Sunburn and licorice tonic effect of Morinda officinalis How has the increase; hypoxia by measuring the time that the anti Processed Morinda Stress capacity has been enhanced.6. Morinda officinalis and its processed products in the study methodology are in line with the relevant provisions of Morinda officinalis and its processed products shall be not less than Nice sugar content 2.0%.Conclusion:1. Sunburn officinalis best salt processing technology through laboratory experiments to enlarge the test results was consistent with the first test to prove that salt processing methods Morinda Sunburn stable and feasible.2. Sunburn Morinda best licorice water processing technology through laboratory experiments to enlarge the test results consistent with the first test results show that licorice Sunburn Morinda officinalis water processing method is stable and feasible.3. Application of column chromatography, preparative TLC, preparative liquid chromatography herbs from Morinda isolated 11 compounds, the use of UV, IR, NMR and MS spectra and other means of identification of the compounds.4. Morinda natural products, salt-broiled product, licorice water broiled product, all have a certain role and anti-stress tonic, and the processed products was more effective than raw materials.5. Nice sugar as an indicator for the development of Morinda officinalis, Morinda and licorice salt water Sunburn Sunburn Morinda viable and stable quality standards can be used to evaluate and control the quality of Morinda officinalis.
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