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Spleen Qi deficiency recipe on the lung tissue of asthmatic rats TLR4, IKKβ expression

Author: ZuoGuangYan
Tutor: LiuChun
School: Liaoning University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with the basis
Keywords: Spleen Asthma Toll-like receptor 4 Nuclear factor -κB inhibitor protein kinase β Interleukin- 8
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 105
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Abstract

Objective: This project by studying airway inflammation in asthmatic rats spleen and lung bronchial tissue TLR4, IKK-β expression changes, explore TLR4, IKK-β expression on airway inflammation in asthma, and to explore Spleen Qi Recipe on TLR4, IKK-β and its effects on the regulation of content of IL-8, further reveal the molecular mechanisms of asthma, spleen Impact on Asthma and Spleen Qi prescription asthma mechanism. Materials and Methods: 84 healthy male Wistar rats were randomly divided into seven groups: normal control group, asthma model group, spleen asthma model group, asthma group, spleen asthma medicine treatment group and western medicine to treat asthma group and western medicine treatment of asthma spleen groups. Spleen group (spleen asthma model group, Chinese medicine treatment spleen asthma group, western medicine spleen asthma group) first use of excessive fatigue, diarrhea bitter cold and diet to establish a combination of factors such as deficiency model, totaling 10 days. Then spleen group with asthma (asthma model group, Chinese medicine treatment of asthma group, western medicine to treat asthma group) using OVA sensitized with excitation method to establish model of asthma. The first 11 days in the experimental model of spleen deficiency is established, the treatment group (traditional Chinese medicine and Chinese medicine treatment of asthma group spleen asthma) Spleen Qi prescriptions given gavage treatment; Western medicine treatment group (western medicine to treat asthma group and western medicine treatment of asthma spleen group) administered dexamethasone treatment, a total of 21 days. Reverse transcription polymerase chain reaction (RT-PCR) detection of lung tissue and IKKβ TLR4 mRNA levels; rats were detected by immunohistochemistry in lung tissue of TLR4 and IKKβ protein levels; enzyme-linked immunosorbent assay (ELISA ) of rats was measured in bronchoalveolar lavage fluid (BALF) of IL-8 levels. Results: 1. Lung tissue mRNA expression level of IKKβ Show: Compared with the control group, spleen asthma model group rats with asthma model IKKβmRNA expression in lung tissue were significantly higher (P lt; 0.01), while the spleen asthma model group was significantly higher than the asthma model group (P lt; 0.01); asthma model group compared with the spleen, spleen asthma medicine treatment group and western medicine spleen lung tissue of asthmatic rats IKKβmRNA expression were significantly lower (P lt; 0.01 ), two treatment groups showed no difference; asthma model group, Chinese medicine treatment of asthma group and western medicine to treat asthma in IKKβmRNA lung tissue expression were significantly lower (P lt; 0.01), the two treatment groups was not differences; 2 protein expression in lung tissue levels of IKKβ Show: Compared with control group, model group and spleen asthma asthma model rats lung tissue IKKβ protein expression were significantly higher (P lt; 0.01), while the spleen asthma model group was significantly higher than the asthma model group (P lt; 0.05); asthma model group compared with the spleen, spleen asthma medicine treatment group and western medicine spleen lung tissue of asthmatic rats IKKβ protein expression were significantly lower (P lt; 0.01), while the treatment group was significantly lower than western medicine treatment group (P lt; 0.01); asthma model group, Chinese medicine treatment of asthmatic lung tissue in IKKβ protein expression were significantly lower (P lt; 0.01), Western medicine to treat asthma group also reduced (P lt; 0.05), whereas traditional Chinese medicine treatment group was significantly lower than the treatment (P lt; 0.01); 3. lung tissue mRNA expression of TLR4 Show: Compared with the control group, spleen asthma model asthma model group rats TLR4mRNA expression in lung tissue were significantly higher (P lt; 0.01), while the spleen asthma model group was significantly higher than the asthma model group (P lt; 0.05); asthma model group compared with the spleen, Spleen asthma medicine treatment group and western medicine spleen asthmatic rats TLR4mRNA expression in lung tissue were significantly lower (P lt; 0.01), two treatment groups showed no difference; asthma model group, traditional Chinese medicine to treat asthma group, Western medicine treatment of asthma in TLR4mRNA lung tissue were significantly lower expression (P lt; 0.05), two showed no difference between treatment groups; 4. lung tissue protein levels of TLR4 Show: Compared with the control group, spleen asthma model group and asthma model lung tissue of TLR4 protein expression were significantly higher (P lt; 0.01), while the spleen asthma model group was significantly higher than the asthma model group (P lt; 0.01); asthma model group and spleen compared to traditional Chinese medicine Spleen asthma group, western medicine spleen asthmatic lung tissue of TLR4 protein expression were significantly lower (P lt; 0.01), while the treatment group was significantly lower than western medicine treatment group (P lt; 0.01); Compared with the asthma model group, traditional Chinese medicine to treat asthma group, western medicine to treat asthma in TLR4mRNA lung tissue expression were significantly lower (P lt; 0.01), two showed no difference between treatment groups; 5. bronchoalveolar lavage fluid IL-8 levels of display: Compared with the control group, spleen asthma asthma model group, model group and alveolar lavage fluid IL-8 levels were significantly higher (P lt; 0.01), while the spleen asthma model group was significantly higher than the asthma model group (P lt; 0.05); asthma model group compared with the spleen, spleen asthma medicine treatment group and western medicine spleen asthmatic rats BALF IL-8 levels were significantly lower (P lt; 0.01), two treatment groups showed no difference; asthma model group, Chinese medicine treatment of asthma group and western medicine to treat asthma rats BALF IL-8 levels were significantly lower (P lt; 0.01), The traditional Chinese medicine treatment group was significantly lower than western medicine (P lt; 0.05). Conclusions: 1. Spleen make asthma lung tissue of rats TLR4, IKKβ mRNA and protein expression levels, the asthmatic rats lavage fluid IL-8 levels increased. 2 deficiency asthma and asthma, lung tissue expression of TLR4 and IKKβ positively correlated, TLR4, and IKKβ expression of IL-8 in BALF were positively correlated Spleen Qi herbs and dexamethasone can be reduced TLR4 , IKKβ thereby reducing the expression of IL-8 levels. 3 Spleen Qi Prescription medicine and western medicine can dexamethasone were significantly lower spleen asthma model group and asthma model group rats lung tissue TLR4, IKKβ mRNA and protein expression levels, lower alveolar lavage IL-8 levels.

Spleen Qi deficiency recipe on the lung tissue of asthmatic rats TLR4, IKKβ expression

Abstract

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