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Chinese medicine research to eradicate Helicobacter pylori (in vitro resistance reaction experiments )

Author: PanHong
Tutor: WangChuiJie
School: Liaoning University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Chinese herbal compound Clarithromycin Metronidazole Helicobacter pylori Drug reaction
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 93
Quote: 0
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Abstract


Objective: In this study recognized anti-the HP better Astragalus, Sophora, dandelion, berberine, the raw licorice flavors drugs for research object, the three optimized by different doses of five drugs prescription anti-HP activity preliminary study, drug-induced reaction experimental study to investigate the resistance, Helicobacter pylori eradication provided a new theoretical basis for traditional Chinese medicine, but also to broaden the scope of application of traditional Chinese medicine. Materials and Methods: Preparation of medium and petri dishes, activate and identification of Helicobacter pylori, the preparation of broth, experimental groups were divided into experimental group (traditional Chinese medicine-induced group, metronidazole induced group, clarithromycin induced group), the control group , of the solvent control group. The multi-step induction method for in vitro drug testing of HP elect clinical resistance high rate of metronidazole and anti-HP efficacy of clarithromycin for synchronization test; observed increase in drug concentration gradually turn medium HP variation of the minimum inhibitory concentration (MIC); species will induce resistant strains of continuous transfer species after 10 Determination of MIC, to determine whether the drug-resistant stable; turn the decreased susceptibility to induced strains on drug-free medium species after three rounds of testing M1C value determination of the the M1C value of the three-optimized set of square decreased susceptibility to metronidazole-induced strain. Results: 1 in a multi-step process of induction culture, not induced by HP to streamline the party to streamline the two parties and streamline the tripartite resistant strains, strains could grow on a medium containing 64 times the MIC of metronidazole, 2 times the MIC of clarithromycin medium strains could grow. 2 during culture, the sensitivity of the strains to metronidazole gradually decreased, M1C values ??increased gradually to 32ug/mL the transferred species; metronidazole-resistant strains (MR) in drug-free medium 10 the MR 'the MIC values ??unchanged; strains gradually decreased sensitivity to clarithromycin, the the M1C value increased gradually to 4ug/mL; clarithromycin-resistant strains (CR) in drug-free medium to 10 species The obtained CR 'MIC values ??unchanged. Streamline the party, to streamline the two parties and the streamlining of the three parties on the MR and CR MIC values ??of their corresponding original strain. Conclusion: This study found that HP three-optimized set of square easy to produce drug reactions, HP prone to drug reaction to metronidazole and clarithromycin. The 2.HP resistance to metronidazole and clarithromycin relatively stable. Metronidazole and clarithromycin-resistant strains remain sensitive three-optimized set of square.

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