Dissertation > Excellent graduate degree dissertation topics show

Denitrifying bacteria screening , identification and enhanced treatment of nitrate wastewater

Author: ChenPeng
Tutor: HuWenRong
School: Shandong University
Course: Environmental Engineering
Keywords: Denitrifying bacteria Filter Phylogenetic Denitrification characteristics Biofortification
CLC: X703
Type: Master's thesis
Year: 2009
Downloads: 772
Quote: 4
Read: Download Dissertation

Abstract


Nitrogen is the cause of water pollution in an important class of pollutants. Currently, the pollution caused by the nitrogen eutrophication has become a worldwide environmental problem. Nitrogen pollution control at home and abroad increasing attention because of their biological nitrogen removal technology quickly and safely favorites. Biological nitrogen removal is the mainstay of denitrifying bacteria, isolated from the environment screened efficiently denitrifying bacterial strains, and mastery of its denitrification characteristics, is to improve the application of denitrifying bacteria key. Soil suspension by dilution - spread plate method from Nansi plant rhizosphere Lo Zhugen gas production was screened a fast, high denitrification denitrifying bacteria LZ-14; separation method using the enrichment culture, respectively, from reeds and cattails rhizosphere soil isolated and screened out three and four denitrifying bacteria. Screened denitrifying bacteria are facultative anaerobic gram-negative bacilli. Origin of gas through the nitrate test also proved screened denitrifying bacteria have good gas production capacity, can be used as biological denitrification bacteria source. Strain LZ-14, compared with the other strains have optimal denitrification capacity, this study focuses on the strain LZ-14 denitrification characteristics were systematically investigated. Strain LZ-14 optimum carbon source was sodium acetate, can use a wide range of carbon and nitrogen sources; suitable temperature 20 ~ 35 ℃ in anoxic conditions denitrification; rapid growth and reproduction; sodium acetate as carbon source, nitrate were investigated potassium as nitrogen C / N and pH on the growth of strain LZ-14 and denitrification processes, the results show that the best C / N is 5, the C / N is 5, the initial nitrate concentration of 150 mg / L in the case, 36h completely removed within the nitrate, TN removal rate of 84.5%; optimum pH range of 7.0 to 8.0, outside this range, strain LZ-14 is difficult to grow and remain off nitrogen capability. Denitrification strain LZ-14 cell growth mainly takes place in the first 12 ~ 36h, and is accompanied by the accumulation of a certain amount of nitrite, nitrate Guinea after 24h can be completely degraded. Amplified by PCR, 16S rDNA sequencing combined with homology analysis to determine the strain LZ-14 taxonomic status (Genbank accession number FJ588910). Strain LZ-14 and Pseudomonas stutzeri closest evolutionary distance, and belongs to the genus Pseudomonas, the genus is a typical feature of the genus with denitrification. Under laboratory conditions, to build a pot experiment simulated wetland investigated dosing strain LZ-14 agents on wetland biological wastewater treatment nitrate strengthening effect. The results showed that after treatment 8d, adding broth intensive group makes new Xue River water contaminated in COD Cr from 33.9 mg / L dropped to 15.9 mg / L, the removal rate was 53.1%; TN from 4.07 mg / L was reduced to 0.98 mg / L, the removal rate was 75.9%. And did not vote bacteria compared to controls COD Cr and TN removal increased by 24.9% and 39.5%, significantly enhanced biological effects, be used as an effective method of wastewater treatment nitrate.

Related Dissertations

  1. Research on Electromagnetic Acoustic Transducer Detection System Based on FPGA,TH878.2
  2. Algorithm Research on SINS/GPS Integrated Navigation Syste,V249.328
  3. Satellite Attitude Determination Based on Gyro and Star Sensor,V448.2
  4. Research on Transfer Alignment of the Missile Onboard the Aircraft,V249.322
  5. Research on Cooperative Orbit Determination in Satellite Network Based on Multi-Agent System Theory,V474
  6. Research on Data Acquisition and Processing Methods for Weapon Evaluation System,TJ06
  7. The Study of Control Algorithm for Automotive Power Window Based on Lin Bus,U463.6
  8. Study of Three-Phase AC Chopper Surge and Its Harmonic Elimination Technology,TM423
  9. Design of Servo Control System with Linear Motor Based on Digital Filter Technology,TM359.4
  10. Maneuver Detection and Tracking with the Radar Rate Measurement,TN953
  11. The Maneuvering Target Tracking Research Based on VRPF,TN957.52
  12. Design and Processing of Hybrid Modulated HF Radar Waveform,TN958.93
  13. Research of High Speed Image Pre-processing System Based on FPGA,TP391.41
  14. Research on Methods of Medical Ultrasound Image Denoising,TP391.41
  15. The Research of 3D Human Motion Capture Based on Reference Points,TP391.41
  16. Research on Visual Detection and Tracking of Mobile Robots,TP242.62
  17. Design of Gyro Stabilized Pod Control System,TP273
  18. Research on Servo Control System of Gyro-Stabilized Platform,TJ765
  19. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  20. Study on Phylogenetic Diversity and Bioactivity of Symbiotic Fungi of Coral,R284
  21. Research on the Theory of the Transmission-line Metamaterials and Their Applications,O441.6

CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > General issues > Wastewater treatment and utilization
© 2012 www.DissertationTopic.Net  Mobile