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The Expression of Seven Proteins in Taxol-sensitive and Resistant Breast Cancer Cells

Author: ZhangYaJie
Tutor: HuangJiangSheng
School: Central South University
Course: General Surgery
Keywords: breast cancer paclitaxel proteomics
CLC: R273
Type: Master's thesis
Year: 2011
Downloads: 11
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Abstract

OBJECTIVE:Through the differential proteins profiling in breast cancer cells of paclitaxel susceptibility and drug resistance by proteomics techniques, to find specific proteins related to drug-resistance and susceptibility with breast cancer and to know more about mechanisms related to tumor drug-resistance in order to provide evidences for selecting clinical drugs for chemotherapy.METHORDS:According to response evaluation criteria in solid tumors(RECIST), we respectively selected 5 patients for susceptibility group and drug-resistance group of chemotherapy on breast cancer paclitaxel through neoadjuvant or primary chemotherapy scheme assisting chemotherapy. The differential protein expressions were carried out among two groups through proteomics techniques, then parts of differential protein were examined through Western blotting.RESULTS:Bidirectional gel electrophoresis fingerprints with high resolution and good repeatability were gained through proteomics techniques. PDQuest 2-DE was used to analyze out that there were 53 protein spots with more than 2 times differences, in which 26 up-regulated and 27 down-regulated in drug resistance group. We also identified 13 differential proteins in which 3 up-regulated and 10 down-regulated in drug resistance group.As some related literatures reported, Western blot was used to validate that 7 proteins related to tumor metastasis, typeⅠcytoskeletal 19 and thymidine phosphorylase are up-regulated in drug resistance group while Heat shock protein beta 1, Keratin, typeⅠcytoskeletal 9, Collagen alpha-2(VI) chain, Vimentin, Actin, cytoplasmicl have down-regulated expression which was consistent with the bioinformatic results.CONCLUSIONS:1. PDQuest 2-DE analyzed out 53 differential protein spots and 13 differential expressed proteins were identified by mass spectrometry. The identified proteins contain chaperone and signal transduction proteins, cytoskeletal proteins and metabolism enzyme and so forth;2. Seven proteins such as Keratin, typeⅠcytoskeletal 19, thymidine phosphorylase were examined by Western bloting and it turned out consistent with the bioinformatic results which suggested that they may play an important role at the process of susceptibility and drug resistance during chemotherapy on breast cancer, but the specific functions need further researches.

The Expression of Seven Proteins in Taxol-sensitive and Resistant Breast Cancer Cells

Abstract

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