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Objective 1 through the detection of colorectal cancer in patients with cancer tissue, paraneoplastic organization, the normal tissue of IGF-Ⅰ R of IGF-Ⅰ and IGF-Ⅱ expression of the situation, analysis of IGF-Ⅰ R, of IGF-Ⅰ and IGF-Ⅱ in cancer tissue. cancer tissue, normal tissue expression differences; 2. analysis of IGF-Ⅰ R of IGF-Ⅰ and IGF-Ⅱ in cancer tissue expression and clinical pathological factors relationship to explore of IGF-Ⅰ R of IGF-Ⅰ and IGF correlation between - II expression and clinicopathologic factors of patients with colorectal cancer; 3. analysis of IGF-I R of IGF-I and IGF-Ⅱ expression in cancer tissues explore IGF system in the occurrence and development of colorectal cancer role. Method 1. Eighth Hospital of Wuhan City is selected by the pathological diagnosis of colorectal primary inpatients, access to medical records, will receive the information fill out the questionnaire in drawing, the survey included demographic characteristics, clinical features, pathological features, etc.; 2. using immunohistochemical SP method detected in patients with colorectal cancer tissues, cancer tissue, normal tissue IGF-Ⅰ R, IGF-I and IGF-Ⅱ expression. Combined with references to set up an anti-dilution gradient, based on the pre-test results to determine an anti-dilution; 3 immunohistochemistry results and negative judgment of the cutoff value determined in accordance with the references, the two blinded reading, the reading results for single people and the consistency test to evaluate the consistency strength between the two. Results 1 IGF-Ⅰ R, IGF-I and IGF-Ⅱ expression in cancer tissues, the positive rate is higher than the adjacent tissues and normal tissues, and a statistically significant (P lt; 0.05), IGF-Ⅰ R, IGF- Ⅱ positive rate differences in the expression of cancer adjacent and normal tissues without statistical significance (P gt; 0.05), the expression of IGF-I in the adjacent tissues positive rate of less than normal tissue, and a statistically significant (P lt ; 0.05). IGF-Ⅰ R of IGF-I and IGF-Ⅱ expression in cancer tissues with the patient's age, gender, tumor size, degree of differentiation, Dukes staging, TNM stage, primary tumor invasion depth of T, regional lymph node metastasis N the correlation was not statistically significant (P gt; 0.05), and the relationship between the expression of IGF-Ⅰ R tumor was statistically significant (P lt; 0.05). 3. Of IGF-Ⅰ R and IGF-Ⅰ in cancer tissue expression of the result of the difference is not significant (P gt; 0.05), of IGF-Ⅰ R and IGF-Ⅱ in cancer tissue express the result of differences in the critical state (P = 0.05). IGF-Ⅰ R and IGF-Ⅰ expression in cancer tissue anastomosis to a lesser extent (P = 0.007), IGF-Ⅰ R and IGF-Ⅱ expression results in cancer tissue anastomosis degree weaker (P = 0.000). Conclusions In this study, only of IGF-Ⅰ R, of IGF-Ⅰ and IGF-Ⅱ next to the cancer tissues, organizations, between normal tissue expression rate observed to a statistically significant difference, the study observed to the IGF system in role in the development of colorectal cancer is not obvious. 2 This study did not observe IGF-Ⅰ R, the positive relationship between the three indicators of IGF-I and IGF-Ⅱ expression with clinicopathological factors, its results are yet to be deeper foundation, clinical and population studies to support and argumentation. The results of the correlation analysis suggest that paracrine or autocrine, endocrine manner in colorectal cancer occurs played a certain role in the development.
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