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Experimental Study on the Intervention of Active Component of Lonicerae Powder on Immune Cytokines of Influenza Viral Pneumonia in Mice

Author: ZuoQing
Tutor: WangXueFeng
School: Liaoning University of Traditional Chinese Medicine
Course: TCM pediatric
Keywords: Flos Lonicerae extract influenza A virus embryonated egg hemagglutination inhibition titer viral pneumonia IL-1β TNF-a
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 253
Quote: 1
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Abstract


Objective:Through honey suckle extraction to Type A influenza virus pneumonia mouse’s therapeutic action and to immune cell factor TNF-α, IL-1βexpression influence experimental study, discussion traditional Chinese medicine honey suckle in immunity regulating process to cell factor influence mechanism.Methods:1.The nasal cavity vaccination Type A influenza virus mouse lung adapts (FM1), the induction mouse forms pneumonia model. Influenza virus RNA and examines its expression with the polymerase chain reaction technology duplication lung organization, union targets and so on tectology, lung index, appraisal mouse pneumonia model.2.The honey suckle extraction fills the stomach to treat the mouse flu virus pneumonia to the medicine. Observes each group of mouse general condition, the lung index, the lung tectology and the influenza virus RNA expression situation, the exposition honey suckle to flu virus pneumonia’s therapeutic action.3.Uses the double immune body to contain filling ABC the coloration - ELISA law examines in each group of mouse blood serum the immune cell factor TNF-α, IL-1βthe expression situation, discusses the honey suckle extraction active constituent initially to the immunity regulating process in the cell factor regulative function.Resyult:1.Model duplication: Infects 5 days later, in the model group’s lung organization examines to IV RNA massive expressions, the lung index advances, the pathology change assumes the mesenchymal pneumonia pathological change.2.Honey suckle extraction to pneumonia mouse’s therapeutic action:(1)Pneumonia mouse’s general condition improvement, the therapeutic action is obvious.(2)Changes the aspect at the protection lung organization pathology: The honey suckle group and the viral zuo group lung index is lower than the model group, has the significance difference (P<0.01);The honey suckle group and the viral zuo group compare the non-significance difference(P>0.05).Under the light microscope observes the lung organization shape to indicate that the honey suckle group and the viral zuo group lung organization pathological change compares the model group obviously to reduce.(3)Suppresses the viral duplication aspect: In the normal group lung organization has not examined to IV viral nucleic acid; Model group after infection the 5th day examines the massive IV RNA expression; The honey suckle group and the viral zuo group are lower than the model group, and the difference has the significance (P<0.01);The honey suckle group compares the viral zuo group to be slightly high, existence difference(P<0.05).3.Honey suckle extraction to pneumonia mouse blood serum in immune cell factor regulation: The normal group may examine few TNF-α, IL-1βthe expression; The model group normal group TNF-α, IL-1βthe expression obvious enhancement, has the significance difference(P<0.01);The honey suckle group and the viral zuo group are lower than the model group, and the difference has the significance(P<0.01);The honey suckle group compares the viral zuo group to be slightly high, existence difference(P<0.05).Conclusion:1.Young age Kunming plants mouse nasal cavity vaccination Type A influenza virus FM1, may form flu virus pneumonia model. With the tectology observation, calculates the lung index and the RT-PCR duplication virus nucleic acid and examines its expression to be possible to appraise flu virus pneumonia model.2.The honey suckle extraction has the certain extent anti-flu virus pneumonia function.3.In flu virus pneumonia mouse blood serum immune cell factor TNF-α, IL-1βexpression obvious upward; The honey suckle extraction to flu virus pneumonia mouse blood serum in the immune cell factor TNF-α, IL-1βthe expression has the regulative function.

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