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The experiment for a large-scale dairy farms in Hefei, epidemiological investigation, research of recessive and clinical mastitis incidence and clinical mastitis pathogen isolation, identification and susceptibility testing to explore cows recessive and the incidence of clinical mastitis, the main type of pathogens, the inhibitory effect of the antibiotics for mastitis pathogens, scientific guidance to clinical medicine, treatment Mastitis disease. Epidemiological investigation shows that the clinical mastitis incidence in cattle in accordance with the left front, left rear, right front, right rear, respectively 5.26%, 6.27%, 4.81% and 7.87% respectively. Before and after the mammary morbidity by chi-square test, its P = 0.2062 gt; 0.05; around mammary morbidity by the chi-square test, its P = 0.798 gt; 0.05 Description four mammary clinical mastitis incidence is not very different . Subclinical mastitis in accordance with the left front, left rear, right front, right rear incidence of 16.92%, 10.70%, 11.11%, and 17.23%, respectively. Before and after the mammary morbidity by chi-square test, the P gt; 0.05; around mammary morbidity by the chi-square test, the P gt; 0.05, to illustrate four mammary subclinical mastitis incidence is not very different. The total incidence of mastitis in accordance with the left front, left rear, right front, right rear incidence of 22.18%, 16.97%, 15.92%, 25.1%, respectively. Before and after the mammary morbidity by chi-square test, P = .4641 gt; 0.05; around mammary morbidity by the chi-square test, P = 0.7488 gt; 0.05, to illustrate the the four milk Chief mastitis incidence difference was not significant. Different month clinical mastitis incidence by chi-square test, the P lt; 0.001; different months of subclinical mastitis incidence by the chi-square test, the P lt; 0.001; different months mastitis incidence by the chi-square test, its P lt; 0.001. Overall, the hot summer and the high incidence of seasonal transition. The mastitis incidence parities also there is a certain relationship between the incidence of mastitis, showing one increased trend with increasing parities. 30 clinical mastitis milk samples were 43 bacteria, including Staphylococcus aureus, Enterobacteriaceae, fungi three categories. Which E. coli 11, accounting for 18.60%; Proteus 2, accounting for 4.65%; Shigella one, accounting for 2.33%; fungal 26, accounting for 60.47%; 4 Staphylococcus aureus, accounting for 9.30%; epidermal aureus 2, accounting for 4.65%. The mastitis caused by fungi. Susceptibility test results showed that the E. coli to ampicillin, ceftriaxone, ciprofloxacin, norfloxacin high sensitivity. The separation of Candida tropicalis sensitive to ketoconazole and nystatin. Staphylococcus aureus amikacin, gentamicin, enrofloxacin, Vanguard ADM IV ceftriaxone, rifampicin high sensitivity. Proteus amikacin, streptomycin high sensitivity. Shigella enrofloxacin, Vanguard ADM IV ceftriaxone, ciprofloxacin, rifampicin, norfloxacin high sensitivity. In the treatment of mastitis in dairy cows, should be based on susceptibility testing results, combination approach to avoid resistance.
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