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SNPs Identification of PRLR Gene and Associations with Egg Production in Goose

Author: YueLi
Tutor: GengZhaoYu；JiangRunShen
School: Anhui Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Goose PRLR gene Cloned and sequenced. SNP Egg production
CLC: S835
Type: Master's thesis
Year: 2008
Downloads: 149
Quote: 0
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Abstract


Animal anterior pituitary secretion of prolactin (prolactin, PRL) has a wide range of biological effects, the PRL is mainly related to the reproductive performance in livestock, poultry the PRL major impact on the nest, thereby affecting the laying performance and other reproductive traits These acts by the prolactin receptor (prolactin receptor, PRLR) regulation. Numerous studies show that: the physiological role of prolactin (PRL) is mediated by the prolactin receptor (PRLR) is located on the membrane of target cells. PRLR in chickens, turkeys, pigeons and other animals have been cloned. Poultry-hold prolactin receptors are closely related. Our local bird species strong nest is one of the main factors of low egg production. In view of this, the candidate gene approach, PRLR gene is closely related to selection on nest traits, comparative genomics, the application Dnastar and PrimerPremier 5.0 software, designed a total of 10 pairs of primers Wanxi White PRLR gene coding region and untranslated region sequences were cloned and sequenced; using PCR-SSCP technology Wanxi White PRLR gene exon 4, 6 and 10, and 5 'UTR fragments were polymorphism analysis and DNA sequencing to detect gene mutation points, and analyzed the correlation between the gene polymorphism and West Anhui white goose egg production. Meanwhile, the Rhine goose the PRLR non-coding sequence made the determination to conduct research. Cloned Wanxi White and the Rhine geese the PRLR gene untranslated region of the sequence 5 'UTR (GenBank accession number: EU534407). Sequence alignment analysis showed that: Wanxi White the PRLR gene non-coding region sequence domestic chickens and ducks homology of 88% and 94%, respectively. Wanxi White and the Rhine goose PRLR gene exon first 4,6 10 and 5UTR for polymorphism detection, results showed that the exon 4,6 amplified fragment polymorphism detected by SSCP not exist, explicit sub 10 is relatively long, more than the total length of 1200 bp, it is designed six pairs of primers amplified fragments detected by SSCP Wanxi White 2 SNP loci; cloned 5 'UTR full-length 455bp, so the design 2 pairs of primers amplified fragment was detected by SSCP Rhine goose (5 'UTR-1), a SNP site. Wanxi White PRLR gene exon 10-2 A181G mutation lead to the encoded Ile64Val amino acid changes, exon 10-3 -/22A deletion mutation. 5'UTR-1 primers to amplify produce two genotypes fragment of the PCR product, after purification, cloning and sequencing results of a -215 / A insertion mutation occurs. Population genetic analysis showed that Wanxi White PRLR10 of-2 primers detected two allele frequencies were 0.7056 and 0.2944, PRLR10-3 primers detected two allele frequencies were 0.8389 and 0.1611, respectively; Rhine geese 'UTR-1 primers detected allele frequencies were 0.8625,0.1375. Wanxi White population egg production records exon PRLR10-2 detected three genotypes were 0.633,0.145 and 0.222, corresponding egg production were 21.46 ± 6.20,22.21 ± 5.95 and 28.97 ± 5.74. Least-squares analysis showed that significant correlation (P <0.05) genotype Wanxi White laying performance. Wanxi White PRLR gene exon PRLR10-detection of polymorphic loci had no significant effect on laying performance.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Poultry > Goose
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