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High oleic and low linolenic acid content of breeding rape quality breeding. Pure lines to further understand the genetic mechanisms of oleic acid and linolenic acid, fatty acid quality improve varieties through genetic improvement, this study selected two oleic acid and linolenic acid content as hybrid parents, take F1 pollen microspore culture building DH mapping population, SSR molecular marker technology, build Brassica napus molecular genetic linkage map of the major fatty acid composition and glucosinolate, protein and oil content traits QTL mapping, the main results are as follows: 1. high oleic (79.88%) low-linolenic acid (2.18%) double low line A A254 as the female parent, low oleic acid (64.07%) of high-linolenic acid (8.01%) A A177 parent Department of double low hybrid, take F1 pollen using small the Microspore Culture 386 DH lines separated monoclonal random selection of 190 DH lines mapping population. A total length of 2330.8cM genetic linkage map was constructed with 463 SSR markers. Mark asked the average map distance of 5.1cM, contains 19 linkage groups, in good agreement with the published map. DH population, 238 (46.03%) markers showed segregation distortion (P <0.05), focused on the N4, N13, N15, N19 linkage group. The positioning of the distribution of the alleles in the DH population: A A254 allele type accounted for 47.66% of the entire group genotypes A A177 genotype accounted for 52.34%. 3 Wuhan Rape quality phenotypic data from 2007 and 2008, 63 QTLs detected in the DH population, 14 2-year repeat QTL detected. Palmitic acid were detected six QTL repeatedly detected two QTL; stearic eight QTLs were detected duplicate detection to three; oleic acid were detected five QTLs detected repeat 1; linoleic acid were detected four QTLs, two 2 years at the same time to be detected; linolenic acid were detected seven QTLs and three duplicate detection, which is located in the N4 and N14 main effect QTL; 6 eicosanoids QTL repeatedly detected two; proteins were detected five QTLs detected a 11 QTLs were detected, oil content and glucosinolate no 2 years at the same time the detected QTL. Oleic major QTL located in the N5 chromosome BNGMS265-BRGMS630 section, explain the genetic variation was 84.63%, the additive effect value 5.03, and for the groups in terms of the traits of oleic acid content of 1:1 bimodal separation that oleic acid content is controlled by a major gene in the population, with less environmental impact. Linolenic acid targeted to two main QTL for which are located on the N4 and N14 linkage groups, respectively, 59.00% and 23.52% of the genetic variation explain. In addition to N5 linkage group positioning of linolenic smaller effect QTL, newly discovered QTL. Of fatty acids grouping and agronomic traits correlation analysis, oleic acid and linolenic acid were found on various agronomic traits and yield related traits not significantly. Description of the study Brassica napus special high oleic and low linolenic fatty acid composition of agronomic traits and yield-related traits are not affected or less affected.
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