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Artemia nauplii as model organisms to establish a new insecticidal activity bioassay methods. Explore the feasibility of Artemia as insecticidal bioactivity screening model by measuring the sensitivity of Artemia of different pesticides, the insecticide of Artemia as biological model; bioassay results by different factors, activity rapid screening method; the Artemia bioassay applications created on 62 kinds of bamboo extract insecticidal activity screening and measurement results of a comparative analysis with traditional aphids biological accuracy of the Artemia biological activity screening model verified. The results are as follows: 1. Artemia measurement results show that the sensitivity of commonly used insecticides: Artemia stage Ⅱ ~ Ⅲ nauplii (exposure to pesticides 24h ~ 48h) is the most sensitive to the insecticidal active substances and Artemia on The sensitivity of the different types of pesticides there is a difference, unless the poor inductive monosultap and destroy Duowei Min, other tested insecticides showed a higher sensitivity. Which, avermectin, fluorine triflumuron Artemia 48h LC50 0.02mg / L, 0.023mg / L, triazophos, pyridaben, chlorpyrifos, fipronil nitrile 48h LC50 0.58mg / L 48h LC50 of 0.72mg / L, 1.04mg / L, 5.97mg / L carbaryl insecticide ring, dichlorvos, cartap metolcarb, deltamethrin 17.48mg / L, 18.12mg / L, 24.69mg / L, 26.91mg / L, 30.90mg / L, 33.33mg / L, while Monosultap and the methomyl 48h LC50 were 443.37mg / L, 535.91mg / L. As can be seen, Artemia nauplii for most chemical pesticides showed higher sensitivity and can be used as a biological model of the insecticidal active substance screening. The impact of different factors on Artemia bioassay results showed that: the Artemia nauplii culture solution salinity, pH, organic solvent, culture temperature, photoperiod, and other factors will affect the Artemia bioassay results. 1 ‰ artificial seawater salinity, Artemia survival rate of only 5.03%, while no one survived in water; culture solution pH 4.5, Artemia survival rate was 28.86%, pH 3, Artemia no survival; temperature higher than 35 ° C will affect the survival of Artemia, 40 ° C, 30.63%, and the temperature rose to 45 ℃, the survival rate dropped to 2.06% Artemia; during the test, a long photoperiod also affect the survival of Artemia, continuous light, Artemia survival rate of only 75.57%; organic solvents such as ethyl acetate, acetonitrile Artemia nauplii toxicity greater concentration of 1%, Artemia survival was 22.6 %, 7.35%, while in the presence of 1% n-hexane, dichloromethane, petroleum ether, toluene, benzene, etc., artemia without a survival. In addition, the size of the volume of the solution to be measured not have an impact on the survival rate of Artemia 1.5mL desirable intermediate amount of the testing process. Established on the basis of the above study, Artemia bioassay methods: culture should be the Artemia nauplii seawater salinity range in 5 ‰ to 120 ‰, the temperature can be controlled at 10 ° C to 35 ° C and pH range of 5 to 10 photoperiod not exceed 18L-6D, the test substance can be used acetone, methanol, ethanol and other organic solvents dissolved organic solvent concentration can be controlled at less than 1%, the Artemia and 1.5mL test solution was added to a 24-hole culture plate culture 48 hours after observed under a stereo microscope the Artemia death number of individuals and the total number of insects, insecticidal bioactive calculated mortality, evaluation of the test solution. Artemia bioassay screening of insecticidal activity of acetone extracts of 62 kinds of bamboo leaves. The results showed that the tested concentration of 1.0g / L bamboo extracts of Artemia nauplii insecticidal bioactive differences. A better biological activity square bamboo, bamboo, bamboo, Guanyin Zhu, bamboo, water bamboo, olive bamboo, short spike bamboo extract, Artemia mortality was 100%, 97.82%, 93.11%, 92.14 %, 86.43%, 84.80%, 84.79%, 75.10%, the LC50 for Artemia 48h: 144.2mg / L and 323.9mg / L and 388.6mg / L and 399.1mg / L and 436.9mg / L, 474.7mg / L, 565.6mg / L, 631.8mg / L; goose feather bamboo 19 bamboo extract, Artemia mortality in 40% to 70%; Pink Sheets bamboo and other 14 kinds of bamboo extract, Artemia mortality in 20% to 40%; Shou bamboo and other 21 kinds of bamboo extract role Artemia mortality of less than 20%, which pointed bamboo, silver Sizhu, and Lei of bamboo, Indocalamus, flowers hanging string and wind The bamboo extract Artemia no biological activity. 4 To verify the accuracy of the of Artemia biological activity screening model the aphids tested biological model, the determination of 62 kinds of the bamboo extract insecticidal biological activity conducted by conventional impregnation method. The results show that: square bamboo, bamboo, green bamboo, bamboo, Guanyin Zhu bamboo extract biological activity, aphid 72h mortality rate was 86.25%, 81.25%, 80%, 78.75%, 74.5%; water bamboo such as the effect of eight kinds of bamboo extract, the the aphid mortality rate in the range of 40% to 70%; such as phenanthrene white bamboo 11 kinds of bamboo extract role, the aphid mortality rate from 20% to 40%; between Jinbi Yu Huang bamboo 38 The role of bamboo extract, the the aphid mortality rate is less than 20%, which pointed bamboo, silver stringed instruments, and hard head Wong Chuk Arundinaria flowers hanging Sizhu five kinds of bamboo extract biological activity on aphids. Seen, the results of the two methods of screening the general trend line, the high activity of Artemia bamboo extract aphids also showed better biological activity of Artemia active low aphid effect is relatively poor, and the majority of activity was significantly higher than that of the extract of bamboo Artemia aphids, prove Artemia biological activity screening model Filter insecticidal active substance is reliable. Artemia as insecticidal activity measurement model organism has a wide variety of sources, the operation is simple, time-consuming short, less the amount of the desired compounds, and other advantages. Bioassay methods as a low cost, high efficiency, Artemia can greatly improve screening efficiency significance of the research and development of new pesticides.
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