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Yellow the Clausena Lansium chemical composition

Author: LiFang
Tutor: XieChen;LuoXiuZhen
School: Peking Union Medical College , China
Course: Pharmacognosy
Keywords: Rutaceae Clausena Yellow Carbazole alkaloids Furocoumarin HPLC
CLC: R284
Type: Master's thesis
Year: 2009
Downloads: 202
Quote: 0
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Abstract


The Rutaceae (Rutacea) Clausena the plants yellow (Clausena lansium) contains a wealth of small molecule compounds , and a wide range of the pharmacological activity . This thesis for the plant Clausena alkaloids and coumarins literature review of the system , including the structure of the compound type , pharmacological activity and spectral law oriented topic extraction, isolation and structural identification of the chemical composition and lay a solid basis. System chemical constituents of this thesis the dried stems of clausenamide , by repeated column chromatography on silica gel , Sephadex LH - 20 column chromatography , preparative thin layer chromatography, preparative HPLC , etc. a variety of chromatographic techniques , from clausenamide Stem the chloride extraction partially separated obtained 10 carbazole alkaloids, a total of 19 of an indole alkaloid , an amide alkaloid , 6 furan coumarin and a sterol compound . Were identified as the basis of physical constants and spectral analytic : Claulansine-A (1), Claulansine-B (2), Claulansine-C (3), Clauseindole (4), Claulansitin (5) ,3-formylcarbazole (6), Methyl carbazole-3-carboxylate (7), O-demethylmurrayanine (8) ,3-formyl-6-methoxy carbazole (9) ,3-formyl-2-hydroxy-6-methoxy carbazole (10), Indizoline (11), 1 -hydroxy-3-formyl-4-prenylcarbazole (12) ,4-methoxy-1-methylquinolin-2-one (13) ,8-hydroxyfurocoumarin (14), Imperatorin (15), Alloisoimperatorin (16), Heraclenol (17) Wampetin ( 18 ) , and of β- sitosterol can ( 19 ) , wherein the compound is -1 , -2 , -3 , -4 , -5, five new compounds , compound 13 is first isolated from the genus , compounds -10 , -12 , -17 for the first time from this species was isolated . Preliminary qualitative and quantitative study of the chemical composition of yellow , the subject of the isolated compounds using HPLC-UV method , HPLC peak identification . Turn to these compounds as a reference , the different plant parts of the yellow stem , root , the chemical composition of the leaf and petiole compare and select compound 15 as an index component to study its content in the stems and petioles . By comparison, it is found the clausenamide the roots and stems of the chemical composition of having a great similarity between them , Step medication having great mutual substitution possibilities clausenamide plant resources protection significance .

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