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The Study on Interaction between Inorganic Nanoparticles and Organic Molecule and Its Application in Biochemistry Analysis

Author: SangYou
Tutor: HuangChengZhi
School: Southwestern University
Course: Analytical Chemistry
Keywords: Platinum nanoparticles Porphyrin Gold nanoparticles Peroxide Polyacrylic acid Polyvinyl alcohol Quantum dots
CLC: TB383.1
Type: Master's thesis
Year: 2010
Downloads: 72
Quote: 0
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Abstract


The inorganic nano-particles as a result of unique physical and chemical properties widely applied. To inorganic nanoparticles effectively used in the field of biochemical analysis, and to explore the interaction between the organic molecules and applied to the detection of small organic molecules and pH. Specific content, including the following three aspects: (1) platinum nanoparticles with electron-rich organic molecules role. Reduction with sodium H2PtCl6 preparation obtained yellow brown nano platinum particles with an α, β, and γ and δ-tetra (N-methyl-3 - pyridyl) porphine (TMPyP-3) interactions found through research citric acid, citrate package be negatively charged platinum nanoparticles the TMPyP-3 J-type occurs gathered its characteristics Soret band and Q-band absorption to reduce the change of fluorescence quenching, scattering signal enhanced CD spectra and dark field imaging. When platinum nanoparticles concentration, degree of aggregation, there are differences in the change in intensity of the optical signal, to lay the foundation for further elucidate the process of formation and morphological studies of porphyrins aggregates. (2) the gold particles for the detection of H2O2. Citrate coated gold nanoparticles is easy to gather in the salt conditions, single-stranded DNA (Single strand DNA, ssDNA) can be to protect the gold nanoparticles, and inhibit their aggregation. General metal ions added exacerbate salt effect, that means weakening the protective effect of ssDNA. It was found that the amount of Fe2 and Cu2 ions can promote ssDNA protected gold plastic salt tolerance aggregation, use a variety of analytical tools of Fe2 and Cu2 Ion affect ssDNA protection mechanism of the gold colloid, may further expand the application field of nucleic acids - the gold nanoparticles sex. Based on the ssDNA and Fe2 ions together to protect the gold nanoparticles, and Fenton's reagent (H202 Fe2) HO · ssDNA cleavage can generate experimental facts, the new method for the development of a method for detecting hydrogen peroxide. In this method, gold particles as probes, using the ssDNA with different concentrations of Fenton reagents, protection of the difference in ability of the gold nanoparticles, and to establish a colorimetric visualization method of detecting hydrogen peroxide. The linear range for the 0.2-8.0μmol L-1, and the detection limit (3σ) of 40 nmol L-1, and the detection of trace biological samples H202. (3) the preparation of the composite nano-colloidal quantum dots and pH sensing. Polyacrylic acid (PAA) to CdTe quantum dots, and then add the polyvinyl alcohol (PVA) as a backbone molecule, the formation of CdTe / Poly (acrylic acid) / Poly (vinyl alcohol) composite nano colloid. PAA with-COOH very sensitive to pH, at pH lt; reunion, the pH gt; 7 Stretch. The study showed that the resulting molecule stretching degree differences, so that the spatial position of the quantum dots in the backbone molecule PVA which relative movement occurs, the fluorescence of the system at this time will change with the change in pH of the medium, PAA different degree of protonation. Thereby establishing a new pH sensor.

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