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The Effects of VEGF、Aggrecanase-1 and TIMP-3 Expression in Rat Mandibular Condylar Cartilage during Early Gowth by Altering Diety Loading

Author: LiZuo
Tutor: YuDaHai
School: Guangxi Medical University
Course: Clinical Stomatology
Keywords: vascular endothelial growth factor(VEGF) aggrecanase-1 Tissue inhibitor of metalloproteinase (TIMP-3) Mandibular condylar cartilage dieting loading
CLC: R782.2
Type: Master's thesis
Year: 2010
Downloads: 35
Quote: 1
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Abstract


Objective: To investigate and verify the hypothesis that VEGF、aggrecanase-1 and TIMP-3 play important roles via affecting the matrix metabolism during altered dietary loading on condylar cartilage in early growth stage.Methods: Forty Sprague-Dawley rats were randomly assigned into two groups: the soft-diet group, which served as the control group (n=20); the hard-diet group, which served as the experimental group (n=20). Five soft-diet and five hard-diet rats were killed at the 24th hour, 48th hour, 7th day, and 14th day after initiation of diet change for hard-diet rats at 28 days of age.The VEGF、aggrecanase-1 and TIMP-3 expression in cartilage at different time points were detected by semi-quantitative RT-PCR.Results: Semi-quantitative analysis revealed that the expression of TIMP-3 mRNA in whole condyle cartilage was higher in soft diet group than in the hard diet groups at the 24th hour after initiation of the experiment ( P<0.05 ), and the aggrecanase-1 mRNA was higher in the hard diet group than in the soft diet groups on the 7th day ( P<0.05 ), and the VEGF in whole condyle cartilage was higher in hard diet group than in the soft diet groups in the 7th day and 14th day (P<0.05).However there were not statistically significantly difference of the expression of VEGF、aggrecanase-1 and TIMP-3 all in the hard and soft diet groups at other times (P>0.05).Conclusion: 1. Results of VEGF、aggrecanase-1 and TIMP-3 expression may indicate a sensitive, temporary and adaptative change in cartilage metabolism in the physiologic range to loading altered by different diets. 2. The change of VEGF positively correlated with loading persistence,while was late for the variance of aggrecanase-1 and TIMP-3 expression.

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CLC: > Medicine, health > Oral Sciences > Oral and maxillofacial surgery > Oral and maxillofacial plastic surgery
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