Dissertation > Excellent graduate degree dissertation topics show

Studies on PVC Membrane Ion-Selective Electrodes Based on Neutral Carriers and Amperometric Immunosensors Based on Layer-by-Layer Self-Assembly Technique

Author: LiuXinLu
Tutor: YuanRuo
School: Southwestern University
Course: Analytical Chemistry
Keywords: Ion selective electrode Neutral carrier Current immunosensor Layer-by-layer assembly
CLC: TP212.2
Type: Master's thesis
Year: 2010
Downloads: 106
Quote: 0
Read: Download Dissertation

Abstract


Ion-selective electrode is a fast, accurate, convenient and selective determination of certain ion content in complex samples analysis test tools. Currently, ion-selective electrodes have been widely used in environmental monitoring, biomedical testing, chemical production, geological testing areas. Poly vinyl chloride (PVC) solvent polymeric membrane ion selective electrode because of its simple operation, low cost, fast response, wide linear range, limit of detection and low current research focus of scientists who have become. Developed a new PVC membrane ion selective electrode lies in the design and synthesis of sensitive carrier. In this article, we have designed and synthesized two schiff base as a neutral carrier prepared two new PVC membrane ion selective electrode. The electrochemical immunoassay sensor is the electrochemical analysis technology and immunoassays combine analytical tool, has been widely used in clinical diagnostics, biomedical testing, environmental monitoring and other fields. The electrochemical immunoassay sensor has a simple, low cost, rapid response, good selectivity and high sensitivity. The key is to build a new electrochemical immunosensor antigen antibody fixed on the electrode. Layer self-assembly technique is based on the electrostatic attraction is fixed with the opposite charge compound multilayer film is formed and thus can be fixed to a method of biological macromolecules, this method to precisely control the composition and thickness of the film, with the advantages of simple operation, diversity, Easy for electrochemical immunosensor Preparation. In this article, we developed a sensitive immunosensor based on the current layer self-assembly technique. First part: the new neutral carrier PVC membrane ion selective electrode. Salicylaldehyde Salicylhydrazone neutral carrier PVC membrane ion-selective electrode aluminum developed salicylaldehyde Salicylhydrazone the neutral carrier of new PVC film the Al3 ion selective electrode. The sensor of Al3 the Nernst potential exhibits excellent response performance. Electrode slope of 20.0 mV / dec, and the linear range of 1.0x10-1 ~ 9.0 × 10-6 mol / L, the detection limit of 7.0x10-6 mol / L. The application range of the pH value of the electrode is 2.5-5.5, with good selectivity of Al3. The response time of the electrode 9s, the electrode potential response after two months, the electrode performance did not decline. By UV - visible spectroscopy, molar conductance method and AC impedance electrode response mechanism. The electrode can be used as the indicator electrode quickly and accurately detect the content of aluminum in real samples of tea, water, rice vermicelli, has a practical value. Malonic hydrazide Schiff base as a neutral carrier the cobalt ion selective electrode salicylaldehyde Propanedioyldihydrazide neutral carrier to prepare a new PVC membrane the Co2 ion selective electrode. The electrode showed good selectivity of Co2 and nearly Nernst potential response performance. The electrode slope 31.1mV/dec, and the linear range of 1.0 × 10-1-3.2 × 10-5 mol / L, the detection limit of 1.0 x 10-5 mol / L. AC impedance techniques to study the electrode response mechanism and electrode as the indicator electrode used in preliminary potentiometric titration of EDTA and Co2 recovery experiment to obtain satisfactory results. The electrode has a simple preparation, fast response and low cost advantages. Part II: Layer-by-layer assembly current immunosensor based on hyaluronic acid and thionine prostate specific antigen current layer self-assembly immunosensor hyaluronic acid (HA) has excellent biofacies Preparation of Layer-by-layer assembly technique for the first time applied to immune sensor capacitive. Electrostatic adsorption of negatively charged HA and positively charged electron mediator thionine (Thi) layers of self-assembled into chitosan modified glassy carbon electrode surface on thionine amino fixed gold nanoparticles (nano -Au) and immobilized prostate-specific antibody (anti-PSA), the new prostate specific antigen current immunosensor. By cyclic voltammetry method (CV), differential pulse voltammetry (DPV) and AC Impedance Spectroscopy (EIS) investigated the electrochemical properties of the immune sensor, and a variety of factors affect the performance of the immune sensor carried out a detailed study . Optimized experimental conditions, the immunosensor a linear range 0.5-4ng/mL and 4 ~ 25ng/mL, detection limit 0.2ng/mL. The immune sensor has the advantages of simple preparation, high sensitivity and good stability.

Related Dissertations

  1. Imidazolium ionic liquids selective electrode and its research activity coefficient,O646
  2. Construction of Multi-responsive Gene Vector Via Layer-by-Layer Assembly,TB383.1
  3. Layer-by-layer Assembly of Nanoparticles for Surface-enhanced Raman Spectroscopy Substrate and the Application in Detection of Organic Molecules,O657.3
  4. Construction and Application of CdTe Quantum Dots Based Electrochemical Biosensors for Catechol,Q813
  5. Preparation of Photo-responsive Microcapsule and Its Application in Photodynamic Treatment,TQ460.1
  6. Construction of a Novel Artificial O2 Carrier Based on Bovine Hemoglobin with Polysaccharides,R318.08
  7. Layer by Layer Assembly of Carbon Nanotubes Modified Biosensor and Its Application,TP212.3
  8. Study on Synthesis, Property and Layer-by-layer Self-assembly of Polyvinylamine,O633.21
  9. Studies on Novel Amperometric Enzyme Immunosensors Based on Assembly of Gold Nanoparticles and Thionine on Nafion Modified Electrodes,TP212.3
  10. Study on DNA Electronics Biosensor and Iodide Electrodes with High Selectivity,O657.1
  11. Study on Metallic Complexes of Schiff Base and Crane Ethane and Apply in Electroanalysis Chemistry,TP212.2
  12. Research on Simultaneous Determination of Lead and Cadmium by Using the Multiple Linear Regression Ion-Selective Electrode,X832
  13. Catalytic Synthesis of N, N-dimethylcyclohexanamine over HX Zeolite,O643.32
  14. Study of the Quick Analysis Method of Cd～（2+）, Cu～（2+）, Pb～（2+） and NO3～- in Wheat Flour,TS211
  15. Research on the Detection Sensor for Total Phosphorus in Environment Water,TP212
  16. Construction of Gene and Drug Delivery Based on Modified Poly (Ethylene Imine),R341
  17. New Methods for Determination of Hydrogen Peroxide,R115
  18. The Design and Implementation of A Water Hardness Online Detector,O657.1
  19. Fabrication and Application of Self-Assembled Nano-Biosensor with Enzyme and Nanoparticles,TP212
  20. Layer-by-layer Assembly of DNA Multilayer Films and Preparation Investigation on DNA Damage,Q523

CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Chemical sensors
© 2012 www.DissertationTopic.Net  Mobile