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Research on Screening and Mutation of Cellulose Degrading Strains

Author: TianZhuang
Tutor: WangLi
School: Jilin University
Course: Microbiology
Keywords: Fungi Cellulose degradation Filter Cellulase activity Mutagenesis
CLC: Q93-33
Type: Master's thesis
Year: 2010
Downloads: 198
Quote: 1
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Abstract


This study are widely collected specimens of nature environment rich in cellulose filter paper as sole carbon source were cultured 53 fungal strains isolated and purified . Preliminary screening of these fungal cellulose Congo red plate method , 10 cellulase production strains . Re-screened by the DNS ( 3,5 - dinitro salicylic acid ) activity assay ( referred to as the DNS method ) 2 FPA activity (filter paper activity, filter paper activity , also known as full enzyme activity ) and CMC enzyme activity (carboxymethyl cellulase, sodium carboxymethyl cellulose enzyme activity , referred to as CMC enzyme activity , also known as endoglucanase activity ) were higher strains . Identified were Aspergillus fumigatus ( Aspergillus fumigatus Number for S69 ) and Fusarium oxysporum ( Fusarium oxysporum , No. S62 ) . Wherein the strain S69 FPA enzyme activity 306.76 U / mL CMC enzyme activity was 548.17 U / mL ; the strain S62 FPA activity 281.55 U / mL CMC activity of 540.7 U / mL . The Aspergillus fumigatus S69 fermentation under different nutritional environment and culture conditions in order to optimize the conditions for enzyme production . Ultimately determine the best conditions for enzyme production of bacteria , culture conditions, in this FPA enzyme activity reached 484.13 U / mL , 57.82% higher than before ; CMC enzyme activity reached 880.51 U / mL higher than before 62.85 % . To further enhance the production of the enzyme activity of the strains , the application of ultraviolet radiation , EMS ( ethyl methane sulfonate ) treatment and both composite role Aspergillus fumigatus S69 mutagenesis . Eventually won six mutant enzyme activity increased , mutant strain S69 - E7 FPA enzyme activity by up to a 16.5 % increase than before mutagenesis , S69 - U3 CMC enzyme activity by up to mutagenesis improve 6.25 % . This study was the ability to degrade cellulose the fungal strains tobacco niger S69 , and the optimum fermentation conditions were optimized , mutation breeding to improve the production of enzyme activity , the basis for future research and production applications strain resources and experimental basis .

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CLC: > Biological Sciences > Microbiology > Microbiology and microbial experiments > Microbiology and microbiology experiments
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