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Cassava Cassava Alcohol is alcohol plant waste , a certain amount of cellulose , hemicellulose and pectin and other substances , the thesis for the purpose of utilization of cassava lees , the following aspects were studied : one to the laboratory now some strains , the use of plant fiber waste to produce cheap and efficient cellulase. Through single factor experiment and orthogonal response surface analysis methods to determine when cassava lees particle size less than 0.425 mm, seed cultivation time 24 h, inoculum size 20% , temperature 30 ℃, initial pH 6.5, (NH4) 2HPO40 .35 g, KH2PO40.3 g, wheat bran, 3 g, lees 7 g, feed water ratio of 1:1.5 , GV solid state fermentation of Trichoderma viride cellulase activity was 56.37 IU / g dry yeast ; when xylose slag particle size 0.15-0.30mm, medium water ratio of 1:2 , the initial pH6.5, xylose residue 3g, bran 7g, (NH4) 2HPO40.35 g, turning song first time was 48 h when Penicillium decumbens solid state fermentation JUA10-1 filter paper activity of cellulase 26.18IU / g dry yeast and the β- glucosidase activity of 75.10 IU / g dry yeast ; when cassava Alcohol 3% , ammonium sulfate 0.5% bran 9% , 10% inoculum , medium volume 50 ml , the Penicillium decumbens JUA10-1 liquid fermentation production of cellulase activity was 3.03 IU / ml, β- glucosidase activity of 1.40 IU / ml . Second, the lees cassava as raw material , the use of cassava lees cellulase enzyme , explores the use of hydrolyzate for ethanol fermentation is feasible. Found that the substrate concentration of 20% to Penicillium decumbens JUA10-1 produced by solid state fermentation solution and spine cellulase enzyme NIP35 Aspergillus spore after mixing for saccharification , 96h dextran conversion rate can be up to 88 % . For subsequent fuel ethanol production basis.
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