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To commercialization sol as raw material, the use of stacked silicon bead method (PICA) synthesized by high performance liquid chromatography (HPLC) with micron mesoporous silica microspheres . The effects of silica , reaction temperature , pH value and other factors influence on the experimental results , the optimal experimental conditions ; and the optimal experimental conditions and amplification experiments were repeated experiments . The synthesized silica microspheres by SEM, particle size analyzer , nitrogen adsorption-desorption by means of the performance test . The results showed that: by optimizing the PICA synthesized silica microspheres smooth surface, good sphericity , the average particle diameter of 3.5μm, the average pore diameter of 17.8nm, the particle size distribution width of less than 2.0 ; surface Si-OH content of 3.444μmol/m2, various parameters meet the requirements of HPLC silica filler ; repeated experiments with good reproducibility . Respectively, commercialization and self- synthetic silica silica as raw material, and CH3 (CH2) 17SiCl3 silylation reaction through bonding , and then by Me3SiCl sealing, preparative reverse-phase C18 silica bonded phase ; the resulting bonded stationary phase merchandise of ODS for comparison, and by chemical titration , infrared spectroscopy, thermal gravimetric analysis prepared by means of bonded silica were characterized , and finally packed columns were evaluated. The results showed that: in PICA silica as a raw material from Preparation method after bonding , the concentration of Si-OH residue 0.280μmol/m2, the percentage content of organic bonding was 11.91% , and commercialization of the relevant parameters rather ODS packing ; the anti- loading phase column and excellent performance. This preliminary study prepared for HPLC silica and C18 silica bonded phase , the development of methods for further optimization of siliceous filler preparation of important guiding significance.
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