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The Study on Antagonism Against Alternaria Solani and Control of Potato Early Blight by the Fermentation of Trichoderma T-115D

Author: GaoLiHui
Tutor: ZuoYuHu
School: Heilongjiang Bayi Agricultural University
Course: Plant Pathology
Keywords: Trichoderma Eggplant tenuis Potato Early Blight Prevention and cure
CLC: S476.1
Type: Master's thesis
Year: 2010
Downloads: 94
Quote: 3
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Abstract

Antagonistic microorganisms is an important means of control of plant diseases, but does not affect the environment and human health, and no resistance. This paper studies the antagonism antagonistic microorganisms Trichoderma T-115D eggplant tenuis its mechanism and potato early blight control effect, the following conclusions: 1. Fermentation broth of Trichoderma T-115D tested 10 pathogens have different degrees of inhibition, suppression of Phytophthora sojae effect the best in the fermentation broth diluted 10-fold inhibition rate of 70.0% on soybean Phytophthora potato early blight fungus, poplar sordida The inhibitory effect of the germs, etc. preferably, the inhibiting rates above 50%, while on the wheat root rot fungus, the Magnaporthe Jundeng less than 50% inhibition rate. 10 pathogens and confront culture of Trichoderma T-115D for the test results show that the rapid growth of Trichoderma T-115D, can occupy most of the space, have a stronger competitive ability than the tested pathogens. Trichoderma T-115D fermentation conditions to determine the most suitable for the growth and metabolism of Trichoderma T-115D medium is the PD optimum fermentation conditions of initial pH 6.0, temperature 25 ° C shaker at 150 r / min, fermentation time for more than 7 d. Potato early blight fungus Trichoderma T-115D dual culture, Trichoderma rapid growth, able to occupy most of the space, and eventually cover the potato early blight fungus, that the competitive effects of Trichoderma antagonistic mechanism, and Trichoderma T- 115D in the potato early blight bacteria to grow on. Trichoderma T-115D potato early blight bacteria dual culture, Trichoderma T-115D spiral wound potato early blight fungus mycelium, prove Trichoderma T-115D parasitic on potato early blight fungus mycelium microscope to see on. Trichoderma T-115D ferment filtrate can be suppressed potato early blight mycelial growth, The inhibitory rate increases with the increase in the content of the fermentation broth. Fermentation filtrate strongly inhibit potato early blight fungus spore germination, fermentation dope potato early blight fungus spore germination inhibition rate of 100%. Trichoderma T-115D fermentation broth has a preventive effect on potato early blight on potato detached leaves the first spray Trichoderma T-115D fermentation broth, the 2 d inoculation potato early blight fungus spore suspension, fermentation dope prevention works best when solani spore inoculum size of 1.0 × 104 cells / mL, fermentation dope control efficiency of 67.0%, when the inoculum size of 2.0 × 104 cells / mL, the control efficiency of 63.3%, when the inoculum size 5.0 × 104 cells / mL, the control efficiency of 62.5%, the greater the amount of inoculation, the lower control effect, the same inoculum size, anti-efficiency decreased with the concentration of the fermentation broth is reduced. First inoculated potato early blight fungus spore suspension spray wooden mold T-115D 1 d after fermentation broth, also has a preventive effect on potato early blight, Early Blight spores inoculum size of 1.0 × 104 cells / mL fermentation dope control effect of 57.1% when the inoculum size of 2.0 × 104 cells / mL, the control efficiency of 50.0%, when the inoculum size of 5.0 × 104 cells / mL, the control efficiency of 42.0%, indicating the fermentation of Trichoderma T-115D liquid have a preventive effect on potato early blight, before inoculation spray better. Soaked potato tubers can prevent the occurrence of early blight on tubers, and the longer the soaking time, the better the results, soaked for 5 h, the control efficiency of 42.0%. 6. Potato Live Leaf preventive effect of the test results show that the fermentation broth of Trichoderma T-115D have a significant preventive effect on potato early blight, the first spray Trichoderma T-115D fermentation broth fermentation dope anti Dongnong 303 efficacy of 41.7% and 13.9% control efficiency of the fermentation broth diluted 10 times, first vaccination potato early blight fungus spore suspension, Dongnong 303 fermentation dope preventive effect of 26.4%, the broth was diluted 10-fold defense efficiency was 6.1%. Watering Trichoderma T-115D method of fermentation broth, can increase the potato plant height, fresh weight and dry weight, watering amount the bigger the better. Watering 250 mL Trichoderma T-115D fermentation broth, and 50 d after plant height of 6.6 cm, compared with the control plant fresh weight increased fresh weight increase of 0.912 g, dry weight increased 0.09 g.

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