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Study on Expression of Orexin Receptor 1 in Rat Pancreatic Tissue with Alimentary Obesity
Author: TianLei
Tutor: ZhaoYuYan
School: China Medical University
Course: Internal Medicine
Keywords: Obesity OX1R Pancreas Orexin 1 receptor antisense oligonucleotide
CLC: R589.2
Type: Master's thesis
Year: 2010
Downloads: 33
Quote: 0
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Abstract
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Objective To identify high fat diet -induced obese rats pancreatic tissue in the expression pattern of increased appetite hormone receptor 1 (OX1R) , to clarify OX1R impact on body weight and metabolic indicators of nutrition obese rats and adjustment mechanism . Method to establish the high-fat diet-induced to establish nutritional obese rat model ; application of micro blood glucose measured whole blood glucose , biochemical enzymatic determination of triglycerides , chemiluminescence immunoassay method for the determination of changes in serum insulin ; Immunohistochemical identification of pancreatic tissue expression of OX1R ; obese rats intracerebroventricular infusion increased orexin receptor 1 antisense oligonucleotide (OX1R-PS-ODNs), to detect its impact on the the obese rat pancreatic tissue OX1R generated . Results After 8 weeks , the obese rats body weight , blood glucose , insulin , triglycerides, total cholesterol higher than that of the control group (p lt; 0.05). Located in the cytoplasm of pancreatic islet B cells , OX1R expression the obese group OX1R the expression levels than normal group reduced by about 16.4 % ( p lt; 0.01 ) . The obese rats intracerebroventricular infusion OXIR-PS-ODNs 72 hours , the expression of pancreatic tissue OX1R a decrease of approximately 34.1 % ( p lt; 0.01 ) . Conclusions pancreatic islet B cells expression OX1R . Hypothalamic OX system can intervene the expression of OX1R nutritional obese rat pancreatic tissue , and participate in the occurrence of obesity and blood sugar , blood lipids, serum insulin metabolic regulation .
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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Metabolic diseases > Lipodystrophy
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