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Study of Ultrasound Hormesis on Dunaliella Salina
Author: XiaoQun
Tutor: DuanShunShan
School: Jinan University
Course: Ecology
Keywords: Ultrasonic wave Stimulating effect Dunaliella salina Overcompensation growth
CLC: Q947
Type: Master's thesis
Year: 2010
Downloads: 49
Quote: 1
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Abstract
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Stimulatory effect of ultrasonic radiation Dunaliella salina Dunaliella algae (Dunaliella salina) , experimental materials , as the ultrasonic generator , ultrasonic cell homogenizer . Ultrasonic time is set , the role of frequency times and the role of the time interval of the three groups of experiments : the first group set ultrasonic continuous radiation 2s , 4s, 8s , 16s , 32s , 64s and 128s7 a radiation time intensity of treatment ; 2 to 64s and 128 s the intensity of the two radiation time , are divided into radiation 1, 2 , 4 times , 8 times and 16 times ( 5 ) role frequency processing ; group 3 selection 64s and 128s2 intensity of radiation time points, with radiation and radiation The interval time is 0s , 2S , 4s and 6s and 8s5 radiation frequency processing . Dunaliella salina absorbance was measured chlorophyll a concentration , the largest solar energy conversion efficiency , practical solar energy conversion efficiency and photosynthetic electron transport efficiency growth and physiological indicators . The results show that : ( 1 ) the effect of ultrasonic radiation time strength has a stimulating effect on the growth of Dunaliella salina . OD value than in the role of a certain intensity ultrasonic radiation , Dunaliella salina algae solution were significantly improved , chlorophyll a content in the culture of the first 5-7 days also increased compared with the control ; (2) ultrasonic radiation frequency processing the Duchenne salt and algae growth pre inhibitory effect on post - promoting effect . Deal with the D. salina damage effect in the first 1-3 days after radiation treatment , the high frequency is less than the low-frequency sub- processing , in the first 4-7 days after treatment , the OD value of the high-frequency treatment group and chlorophyll a content significantly higher than the control and low-frequency sub- treatment group ; ( 3 ) ultrasonic time interval treatment significantly inhibited the growth of Dunaliella salina effect , there is no significant difference in the inhibitory effect of ultrasonic different intervals between the treatment groups ; ( 4 ) the appropriate ultrasonic treatment enables Dunaliella salina increase in the recovery of the growth phase of the largest solar energy conversion efficiency , the actual solar energy conversion efficiency and photosynthetic electron transport efficiency . Thus , the appropriate role of the ultrasonic radiation can stimulate Dunaliella salina ultra compensation growth process .
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CLC: > Biological Sciences > Botany > Plant Biophysics
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