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Study on the Application of Magnetic Microspheres Based Chemiluminescent Immunoassay

Author: DingZuoJie
Tutor: GuHongChen；XuHong
School: Shanghai Jiaotong University
Course: Biochemistry and Molecular Biology
Keywords: Magnetic microspheres Immune magnetic ball Chemiluminescence Immunoassay hCG Non-specific adsorption
CLC: R155.5
Type: Master's thesis
Year: 2010
Downloads: 360
Quote: 0
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Abstract


Chemiluminescence immunoassay technology is established immunoassay technology in the late 1970s, it is a sensitive chemiluminescent reaction immunoassay technology combining a detection technology. Chemiluminescence immunoassay technology has both the specificity of the immune response and high sensitivity chemiluminescence reaction markers also have a long shelf life, wide linear range, can realize the advantages of full automation, and thus in many fields have been widely applications, especially in clinical immunoassay dominate, such as the detection of hepatitis, the detection of tumor markers, markers of myocardial injury, as well as food pathogens detection. In recent years, magnetic microspheres as a novel solid-phase support has been widely used in the radioimmunoassay, ELISA and chemiluminescent immunoassay. In this paper, prepared in our laboratory the surface silicon oxide of the carboxy-modified magnetic microspheres as an immunoassay solid phase carrier using the EDC / NHS activation of the carboxyl group of the surface of the magnetic microspheres, and on this basis will be anti-β-hCG antibodies are immobilized on The magnetic ball surface preparation immunomagnetic microspheres. Then prepared by immune magnetic ball carrier based chemiluminescence immunoassay method based on magnetic microspheres, and to study the factors that affect the chemiluminescence immunoassay. Established based on magnetic microspheres chemiluminescence immunoassay method to complete the detection of β-hCG in the sample simply 45min. Experimental batch error is less than 13%, the batch error is less than 10%. The minimum detectable magnetic chemiluminescence immunoassay is is 1.42mIU/ml, linear range 0-750mIU/ml. Magnetic chemiluminescence immunoassay method value than the limit of detection of magnetic ELISA 2.36mIU/ml, the linear range 0-500mIU/ml has been greatly improved. Different magnetic microspheres is applied to an already constructed magnetic chemiluminescent immunoassay systems, the relationship between the different surface structures to study magnetic microspheres chemiluminescence immunoassay results. The magnetic particle size of the microspheres, coating a matrix material, the functional group structure and the density of the detection signal. In addition, the magnetic chemiluminescence immunoassay method has been established on the basis of the establishment of the non-specific adsorption capacity of the evaluation of magnetic microsphere method. Were evaluated for different particle size, the magnetic microspheres coated matrix material in the detection of chemiluminescent immunoassay systems the background signal, and the immunomagnetic microspheres performance of the non-specific adsorption of serum for further research, development suitable for the immune detection of the magnetic microspheres provide the basis of theoretical and experimental evidence.

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CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Food hygiene and food inspection > Food hygiene and inspection
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