|
Drought are important factors that affect plant growth under drought stress conditions , plant metabolism changes in physiological activity hampered affected at various stages of growth and development , plant wilting phenomenon , drought tolerance through genetic engineering to improve the species is to solve effective method for this problem . AsEXP1 gene is isolated from extreme heat grasses A. scabra been extended protein gene expression of a large number of high temperature stress . Extended Proteins induced loose wall, increased cell stretching, to relieve the tension of the cell walls , thereby enhancing the ability of plants to resist high temperatures . Thus affecting plant to adapt to adversity by regulating cell wall stretch due to expansion of the protein , the expression of the speculated the heat resistance gene AsEXP1 the same can enhance the resistance of plants to drought , which requires in-depth study of AsEXP1 gene . Order of further study AsEXP1 gene function , this study was cloned from bentgrass PenA-4 regulatory regions of the AsEXP1 gene upstream 962bp 5 'end , and connect it with the GUS gene transformed rice callus to do functional analysis , having promoter activity by high temperature regulation . Analysis AsEXP1 gene promoter sequence comprising basic transcription element of the promoter TATA box and AGGA box, TATA box core area of ??multiple cis-acting elements of GA, ABA and drought control , inferred AsEXP1 gene by GA, ABA, drought stress induced expression . In addition , build AsEXP1 gene overexpression vector in-depth analysis of its functional basis for the study of plant tolerance mechanism for follow-up stretch protein regulation , stress environment grassland productivity improvement strategies result in a new strategic direction .
|