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The purpose of breast cancer is one of the most common malignancy in women. For breast cancer in China is low-incidence countries, but its incidence rate has increased year by year, has leapt to the China's first female malignancy. Seek efficiency and low toxicity of anticancer drugs and treatment has been a research focus of the scientific community. Scorpion venom (scorpion venom, SV) from venom the Heliconia poison Cystadenomatous emissions. Scorpion venom heat resistant protein used in this experiment (scorpion venom heat-resistant protein, SVHRP) SV special treatment process, separation and purification, to get the heat a certain molecular weight range of effective components, significantly reduce its toxicity, efficacy obvious enhanced. Studies have shown that for a variety of tumor cells, SVHRP inhibit. The purpose of this project is to study the killing effect of SVHRP vitro cultured human breast cancer MCF-7 cells, and to explore its mechanism of action, provided the experimental basis for the development of anticancer drugs and clinical application of the anti-tumor SVHRP. 6,12,24,48 h different concentrations SVHRP processing of the logarithmic growth phase in vitro cultured human breast cancer MCF-7 cells, the application of methyl thiazolyl tetrazolium bromide (MTT) colorimetric assay absorbance ( OD) values ??calculated inhibition rates, find the strongest inhibition of cell SVHRP concentration and duration of action, as the optimal concentration used in other experiments, specifically: by flow cytometric analysis of cell cycle changes, immunocytochemistry ( SP) was used to detect the expression of Bcl-2 and Bax protein expression. Results 1.MTT results: SVHRP growth of MCF-7 cells significantly inhibited, and increasing dose, prolonged duration of action, inhibition gradually increased. Which to the 0.1μg/ml of SVHRP role 24 h of growth inhibition in MCF-7 cells most significantly, the growth inhibition rate of 56.4%. Flow cytometry results: DNA histogram plus drug group G1 peak the hypodiploid peaks (apoptosis peak); quantitative analysis shows SVHRP group apoptosis rate 0.1,0.01 μg / ml concentration respectively 14.18%, 11.39%, compared with 6.18% of the untreated control group differences were statistically significant (P lt; 0.05); compared with the untreated group that SVHRP group in the G1 phase of the cell to reduce in the S phase of the cell increased. Immunocytochemistry results: 0.1, 0.01, μg / ml of SVHRP role for 24 h in MCF-7 cells of Bcl-2 expression positive rate was 10.64% and by 35.16%, Bax were 39.07%, 35.15%, and the control group were Bcl -2:39.10%, Bax: 21.21%. Conclusions SVHRP on human breast cancer cell line MCF-7 growth inhibition and induction of apoptosis exists, its mechanism of action may be related to the Bcl-2 regulation of Bax gene expression related.
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