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In this study, the national \and the herbs expression of various factors, the proposed \effect constitute the three elements of the effects of the medicine. Among them, the body is drugs play a role in the carrier, is the the efficacy output of relying, constitute an important medicinal elements. Characterize different state of the body with a variety of physiological indicators, as well as how different states affect the the drug biological effects of expression is one of the elements of research. Cold-dispelling pungent and hot cinnamon the Weixin warm, normalized kidney, liver, heart, spleen, and became the subject of one of the tested drugs. Its in vivo metabolic status of different body state to do research, provided the experimental basis for elaborate medicinal essence. Cinnamon (Cortex Cinnamon) is the dried bark of Lauraceae cinnamon Cinnamon cassia Presl, Chinese medicine, cinnamon acrid, sweet, hot, owned by the kidney, liver, heart, spleen, yang make up the fire, Return Fire source, and cold pain , the effect of promoting blood circulation, clinical commonly used in the treatment of impotence, cold uterus, waist and knee Leng Tong, kidney for asthma, the confidants Lengtong Deficiency vomiting and diarrhea, Han Shan, dysmenorrhea, containing volatile oil, flavanols and its multi- trimer, diterpene compounds, tannins. Cinnamon is more concentrated in the chemical composition and pharmacological studies, the cinnamon body analysis is mainly concentrated in the compound in vivo studies. This article is aimed at from the point of view of the chemical composition, the presence of metabolic differences in different states of the body to explore the same chemical composition of the same Chinese medicine. The experimental part of the first chapter prepared cinnamon oil investigated the stability of cinnamon oil was determined by HPLC method, the results show that cinnamon oil is stable for three weeks, and the relative content of cinnamaldehyde in cinnamon oil was determined by GC-MS method was 82.86% . The second chapter confirmed ingredient of cinnamon oil into the blood, the results show that its mainly into blood components of cinnamic acid, detection of the Rats samples to be measured, the results show that the brain can only be measured trace amounts of cinnamic acid, not can reach the limit of quantitation, could be detected in the blood, heart, liver, spleen, lung, kidney, cinnamic acid, to determine the blood, heart, liver, spleen, lung, kidney measured. Determine cinnamic aldehyde (purity gt; 99%) as the research object, starting from the active ingredients of cinnamon perspective of its different body status of rats in vivo pharmacokinetics studies and tissue distribution studies. Chapter established blood, Determination of cinnamic acid in the heart, liver, spleen, lung, kidney tissue samples, the results showed that the method with good linearity, accuracy, precision required, samples 12h room temperature stability, samples were refrigerated at -20 ℃ were good stability, and the 20th -80 ℃ Refrigerated stability of the 7th, the detection limit was 1.2ng (S / N ≥ 3), the limit of quantification 4ng (S / N ≥ 10), to meet the test requirements. And cinnamaldehyde gavage in normal rats to a pre-experiment, cinnamic aldehyde into the body quickly metabolized into cinnamic acid, and quickly reach the maximum blood concentration, reduce and eliminate gradually within 3 hours, in the organ to eliminate quickly to time in the lungs the longest and have the highest concentration. The fourth chapter examines cinnamaldehyde in normal rats, Deficiency rats and Hot rats in vivo pharmacokinetic differences of the process, with a the hydrocortisone copy Deficiency model, dexamethasone copy Xure model, normal rats given saline, after 14 days, the group of single-dose cinnamaldehyde at different time points after the blood and organ determination and were drawn to the concentration-time curve, blood samples data after DAS2.1.1 pharmacokinetic software processing, the use of non-compartment model parameters for analysis, the normal group and Deficiency group Xure group AUC (0-t), AUMC (0-t), MRT (0-t), t1/2z, Vz / F.CLz / F parameters. The analysis results show that cinnamic aldehyde is less than the normal group Deficiency rats in vivo uptake and elimination rate faster than the normal group; uptake of cinnamon the aldehyde in Xure group rats was slightly less than the normal group, eliminating the speed with normal group no significant difference. Normal group organization organ content distribution: lung gt; liver gt; kidney gt; spleen gt; heart; Deficiency model group organization organ content distribution: (liver, lung) gt; kidney gt; spleen gt; heart; virtual The thermal model group organization the organ content distribution pulmonary gt; spleen gt; kidney gt; liver gt; heart. It can be seen that the rat model of cinnamic acid in vivo tissue distribution. Summary of the experiments of this thesis: (1) confirm the cinnamon oil than a larger cinnamic acid into the blood component (2) into the blood components investigated cinnamic aldehyde in rats of different body state pharmacokinetics and tissue distribution results to rats modeling affect cinnamaldehyde vivo metabolism and tissue distribution (3) to establish the HPLC determination method of cinnamic acid in vivo in rats.
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