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Experimental Research on Regutating the Expression of PTEN、PCNA and Fas by Chinese Recipe QiYiYi Ganjian

Author: ShiGuoJuan
Tutor: ZhangHong
School: Dalian Medical University
Course: Chinese medical science
Keywords: The Qi Yi Yigan fried Transplanted liver cancer PTEN PCNA Fas
CLC: R285
Type: Master's thesis
Year: 2010
Downloads: 54
Quote: 0
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Abstract

Objective: To study the of stilbene the Yi yigan fried inhibition of transplanted liver cancer in mice , and its impact on the expression of PTEN, PCNA and Fas , to further explore the possible mechanism of its anti-tumor . Methods: in mice bearing H22 transplanted hepatoma , and the establishment of a saline control group , the the stilbene the Yi yigan fried treatment group and 5-Fu treatment group , compared with 15 days after the strength of the inhibitory effect of each treatment group : Calculate the thymus index and spleen index ; using immunohistochemical techniques , image analysis technology for the quantitative detection of comparison groups of transplanted liver cancer tissue PTEN, PCNA and Fas expression . Results : 1 in all treatment groups of mice transplanted liver tumor tissue growth inhibition assay results are as follows: saline control group ( n = 8 ) tumor weight was 0.91 ; the stilbene the Yi yigan fried treatment group, tumor weight 0.57 , the inhibition rate significant difference ( p lt; 0.01 ) ; 5 - Fu treatment group, tumor weight 0.45 inhibition rate was 51.00% , compared with saline , a significant difference ( p lt ; 0.01) was 37.00% , compared with saline . Each treatment group thymus index , spleen index calculated results are as follows : saline control group, the thymus index was 24.52 ± 6.03, spleen index was 81.37 ± 22.39,5-Fu treatment group thymus index was 22.80 ± 8.89 , spleen index thymus index 53.82 14.15 stilbene the Yi yigan fried treatment group was 39.04 ± 12.16, the spleen index to 89.50 ± 32.98 , compared with the control group and 5-Fu treatment group showed a significant difference ( p lt; 0.05 ) . Using immunohistochemical techniques to observe the transplanted liver cancer tumor tissue of PTEN expression of Fas , three groups were expressed . Image analysis and variance analysis , PTEN , upregulation of Fas Qi Yi Yigan fried treatment group compared with the saline control group , a significant difference ( p lt; 0.05 ) . Using immunohistochemical techniques to observe the PCNA expression in the transplanted liver cancer tumor tissue , were expressed in three groups . Image analysis and analysis of variance showed downregulation of PCNA the stilbene -ju Yigan fried treatment group compared with the saline control group , a significant difference ( p lt; 0.05 ) . Conclusions : 1 . Stilbene the Yi yigan fried transplanted liver cancer in the tumor tissue of the mouse H22 significantly inhibited . 2 the stilbene the Yi yigan fried can increase the weight of the tumor-bearing mouse thymus and spleen .

Experimental Research on Regutating the Expression of PTEN、PCNA and Fas by Chinese Recipe QiYiYi Ganjian

Abstract

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