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The Amino-Modification on the Surface of Magnetic Fe2O4/SiO2 Support and the Immobilization of Laccase on IT

Author: WeiDongXue
Tutor: WangHongXia
School: Harbin Normal University
Course: Physical and chemical
Keywords: Immobilized laccase Fe3O4/SiO2 Surface amino modification Degradation of Procion
CLC: Q814
Type: Master's thesis
Year: 2010
Downloads: 104
Quote: 1
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Abstract


Laccase is a multi-copper proteins, with a strong oxidizing ability, the degradation of phenols, aromatic amines, polycyclic aromatic hydrocarbons, and corresponding derivatives of a variety of biodegradable organic pollutants can be oxidized. The laccase therefore has important applications in industrial organic wastewater treatment value. Free laccase, immobilized laccase in the enzyme recovery, reuse and improved stability with respect to a great advantage. There are a lot of carriers, such as activated carbon, silica, polystyrene microspheres, chitosan, in the the laccase immobilized aspect has been very good application. The magnetic material is a novel laccase immobilized materials the laccase system separation can be achieved by an external magnetic field, the procedure is simple, low-cost operation, there are good prospects for application development. For this reason, people such as the magnetic material Fe3O4 introduced immobilization carriers and laccase immobilized by covalent - coupled method. In this paper, the superparamagnetic Fe 3 O 4 / SiO 2 nanoparticles as a carrier-supported free laccase, in order to achieve the stability of the laccase solid overload and rapid separation. Of Magnetic Fe 3 O 4 / SiO 2 of the functional groups of the carrier surface is hydroxy, in order to require the carrier together with the stability of the free laccase key amino-modified, to the amino group of the surface containing functional groups. In the course of conducting the process of the amino-modified, amino-modified impact conditions and an amino number the the laccase immobilization and activity recovery. Have been reported in the literature to use more organic solvent such as toluene, glycerol and under high temperature conditions, under relatively mild conditions, we tried to realize the magnetic carrier amino modified, using ethanol / water system at room temperature under the conditions of the surface of the carrier amino modified investigated APTES dosage and reaction temperature on the the immobilized activity recovery after the impact, in order to optimize the amino modified conditions, then glutaraldehyde cross-linking agent laccase immobilized explore most of the immobilized laccase appropriate conditions, and the study of enzymatic properties and catalytic performance. Through the transmission electron microscope (TEM), infrared absorption spectroscopy (FTIR) and UV - visible absorption spectrum (UV-Vis) and other means for Immobilizing the sample before and after the characterization. The results show that, under relatively mild conditions to achieve the modification of amino group on the magnetic carrier, laccase in of Magnetic Fe 3 O 4 / SiO 2 carrier successfully immobilized with high operational stability the best solid load conditions that: the magnetic Fe 3 O 4 / SiO 2 carrier 100 mg, glutaraldehyde concentration of 2.5% immobilized identified as 10 h, the initial paint enzyme concentration of 0.6 mg / ml, 55.2% of the activity recovery force. Laccase immobilized before and after the reaction to the optimum pH and optimum temperature is not changed, immobilized laccase has a good stability in operation, and after the immobilized laccase Procion azo dye capable of degrading and 20 ° C for 2 h, the decolorizing rate was 54.7%, the mediator ABTS, bleaching time is shortened to 30 min, the decolorization rate reached 84%, ABTS laccase degradation Procion dyes have a significant role in promoting.

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