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Sarcophagidae (Sarcophaga peregrina) Diptera Sarcophagidae insects. Characterized by chest with pale gray and black stripes , abdomen light gray and dark gray plaid , their breeding fast. Sarcophagidae is a great depth development and utilization of renewable resources insects . In addition to pupal skin ( shell ) is a high-quality raw materials insect chitosan , the cytoplasm is rich in protein , is a variety of active protein repository. In this thesis, Sarcophagidae cytosolic proteins were analyzed and studied all of which are antibacterial and antioxidant components . One research established Sarcophagidae cytosolic protein separation and extraction of the whole experimental program . Using water extraction, alcohol , salt mention, such as alkali extraction method , were given water-soluble protein , gliadin , globulin , alkaline water- soluble protein and insoluble protein , as measured by Brandford content of various proteins , including albumin , small peptides were 41 % and 33% , gliadin protein content at least 3.98% , for the whole protein Sarcophagidae cytoplasmic accumulation of raw data analysis . The two research content , the use of two-dimensional electrophoresis technology to establish a wholly Sarcophagidae cytosolic protein electrophoresis pattern experiments. Focus on exploring all Sarcophagidae cytoplasmic protein two-dimensional electrophoresis working conditions. Initially solved the cytoplasm Sarcophagidae a lot of fat , pigments and polysaccharides on the two-dimensional electrophoresis of serious interference problems , in order to obtain an ideal way Sarcophagidae whole protein electrophoresis patterns and proteome research foundation. The three research content , using acid method for the separation of Sarcophagidae cytoplasmic protein oxidation - hydroxy scavenging identification. Its antioxidant obtained basic data , albumin and globulin hydroxyl radical scavenging rate can reach 80%, the other hydroxyl remove protein components can reach more than 45% ; purified by chromatography step antimicrobial peptides , electrophoresis showed a single band , peptide molecular weight less than 14.4KD. Using filter paper method for antibacterial activity of proteins extracted explore , discover its Mucor , Rhizopus significantly inhibited ; cytosolic proteins as Sarcophagidae beauty care, animal natural food preservatives , etc. provide the basis for the development and utilization .
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