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China Offshore common jellyfish and ( worms Rong ) like DNA barcoding and phylogenetic studies

Author: WangRenCheng
Tutor: SunSong
School: Graduate School , Chinese Academy of Sciences ( Institute of Oceanography )
Course: Marine Ecology
Keywords: DNA barcoding Phylogeny Jellyfish Amon Suborder
CLC: Q951
Type: Master's thesis
Year: 2010
Downloads: 128
Quote: 0
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Abstract


DNA barcoding (DNA barcoding) technology is the use of a short DNA fragment for species identification and characterization of molecular biology techniques. It can be used for species identification, a clear biological life history stages, there may be community structure and phylogenetic studies of effective means. For most metazoans, the mitochondrial cytochrome oxidase first subunit (COI) DNA barcoding can be used as a suitable gene. DNA barcoding technology applications intraspecific differences in the key point is to be significantly smaller than the differences between species. Our Chinese offshore jellyfish and  classes conducted some common types of DNA barcoding analysis, and  class conducted phylogenetic studies. This paper is divided into two parts: one, like jellyfish jellyfish DNA barcoding analysis of number of species widely distributed in the zooplankton community occupies a very important position. Due to its highly diverse forms, the great differences in larval adult characteristics, resulting in a traditional morphological classification encountered in the application of a larger obstacle; addition, the first mitochondrial cytochrome oxidase subunit (COI) in jellyfish class Identification of the just started, its identification accuracy there is still a controversy. Therefore, this study selected the COI, mitochondrial large ribosomal subunit (16S) and nuclear ribosomal RNA genome spacer (ITS) of three kinds of fragments identified comparing its effectiveness as a jellyfish. COI sequences totaling 40, intraspecific differences from 0 to 0.046, from 0.066 至 interspecies differences 0.173; 16S sequences totaling 18, intraspecies variation from 0.003 to 0.010, from 0.118 至 interspecies differences 0.273; ITS sequences totaling 17, intraspecific differences are 0, differences between species from 0.138 to 0.344. In this experiment groups involved in the three sequences can effectively distinguish different kinds. However, as the number and type of analysis does not cover most of the jellyfish sub-group categories, we can not confirm the sequence which is more effective. Two,  class and phylogenetic analysis of DNA barcoding research  class as an important marine food chain in a ring, there are many scholars at home and abroad have been studied. But no one has its DNA barcoding and phylogenetic been studied. This study combines the resulting sequences and the NCBI website COI related sequences totaling 78 sequences  class analysis of DNA barcoding, intraspecific differences from 0.002 to 0.031, between species from 0.083 to 0.410, intraspecific and interspecific differences were not formed overlap. The phylogenetic neighbor-joining tree of all individuals of a species can form a monophyletic branches. The results showed that, COI gene DNA barcoding as  class research effective tool. Measured  suborder (Hyperiidea) 16 个 species partial 18S rDNA sequences from GenBank retrieved 11  class partial 18S rDNA sequence homology comparison, explore its phylogenetic relationships. By the Bayesian phylogenetic tree showed that: in the amphipod, Gammarus suborder (Gammaridea) and straw insect suborder (Caprellidea) closer relationship, clustered into one,  suborder yes yes the branch sister group monophyletic. Shen  superfamily (Phronimoidea), Lu  superfamily (Vibilioidea) and wide-leg  total families (Platysceloidea) constitute a monophyletic  suborder, road  superfamily (Vibilioidea) and wide-leg  General Branch Road mutually sisters group.  is total and phylogenetic branch, near Shen  Branch (Paraphronimoidae) away from the road  total families alone to form a.

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