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Our previous work has demonstrated the the TaSI1 and TaSC gene expression induced by salt, and prove that these two genes can improve salt tolerance in Arabidopsis by heterologous transformation experiments. The which TaSI1 obtained by the method of two-dimensional electrophoresis, salt-tolerant materials RH8706-49 and the salt-sensitive material RH8706-34 salt-induced expression of the genes were up-regulated. TaSC genes by cDNA-AFLP G09-94, differential cDNA sequence, based on EST than silico cloning. Closer evolutionary relationships of rice and wheat, the experiment by over-expression of these two genes in rice, respectively, to further study its salt tolerance. The selection of pTCK303 implementation of expression binary vector were constructed of the pTCK303-TaSI1 pTCK303-TaSC over-expression vector, and further transformation of rice. We know that the homologous genes in vivo the functions are substantially the same, therefore we carried out at the NCBI website homologous than respectively found of wheat TaSI1 and TaSC homologous gene, and are named as OsSI OSSC. Further experiments to study the function of these two genes in rice through the means of RNAi, to prove TaSI1 and salt tolerance gene TaSC the other hand. We the selection pTCK303 as implementation of the the RNAi strategy of dual carrier. A specific period of cDNA fragments as RNA interference, respectively the name OsSIi and OsSCi selected in the OsSI, and OsSC gene sequencing twice digested connection RNAi fragment forward reverse the two incoming pTCK303 plasmid after , completed pTCK303-OsSIi, pTCK303-OsSCi construction. Building completed in the binary vector through Agrobacterium-mediated transformation of rice culture of selective culture differentiation culture T0 transgenic plants. Transgenic lines are as follows: the pTCK303-OsSIi for the 27 a, pTCK303-TaS11 20, pCAMBIA1300 ProTaSI1 :: GUS 11, pTCK303-OsSCi 14, pTCK303-TaSC 19, pTCK303 empty vector 4 . Real time-PCR method to detect the the RNA OsSI, and OsSC gene transcription interference strains this the two turn OsSI gene transgenic lines line i-3, i-4 decreased by 50%, 70%; OsSC gene of three transfected gene strains line i-1, i-2, i-20 decreased by 20%, 25%, 50%, the RNA interference experiment is successful. Detected by semi-quantitative RT-PCR TaSI1, and TaSC gene transcripts overexpression plants, which TaSI1 gene four transgenic lines OX-1, OX-2, OX-5, OX-9 mRNA volume significantly rise; TaSC gene OX-10 mRNA quantity increased significantly, OX-1, OX-2 two transgenic lines also expressed the TaSC genes, mRNA levels rise. Turn salt tolerance analysis of T1 generation transgenic plants TaSI1 the gene three over expression plants OX-2, OX-5, OX-9 than the control growing well, and the survival rate is about 20% higher than the empty vector, which is the mRNA quantity of the previously detected over-expressing plant results are consistent; the Tasc gene three overexpressing plants OX-1, OX-2 two lines with control growing and there is no significant difference, and the survival and empty vector Similar, OX-10 survival rate of less than 20% higher than the control, and the result is also consistent with the previously detected mRNA quantity of the over-expressing plant. T1 generation OsSI, and OsSC gene RNAi plants salt tolerance analysis results show that the two strains OsSI RNAi i-3 and i-4 growing significantly worse than the control, and the survival rate is lower than that of the control; the OsSC gene RNAi three lines i-1, i-2, i-20 survival rate was significantly lower than the control, the degree of expression of the two genes phenotype and mRNA is consistent. At the same time by histochemical localization TaSI1 gene analysis showed that the gene was expressed in the callus, stems, internodes, ligule, stamen, buds, roots and leaves expression.
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